%0 Journal Article
%T Validation of the Use of Dried Blood Spot (DBS) Method to Assess Vitamin A Status
%A Elham Fallah
%A Seyed Hadi Peighambardoust
%J Health Promotion Perspectives
%D 2012
%I Tabriz University of Medical Sciences
%R 10.5681/hpp.2012.021
%X Background: Vitamin A deficiency is an important dietary deficiency in the world. Thus, the ne-cessity of screening for deficient populations is obvious. This paper introduces a fast, cheap and relatively reliable method called ¡°dried blood spot¡± (DBS) method in screening the deficient populations. The validity of this method for retinol measurement was investigated.Method: The ¡°precision¡± and ¡°agreement¡± criteria of the DBS method were assessed. The preci-sion was calculated and compared with those of plasma using F-test. The agreement was eva-luated using Bland-Altman plot.Results: The imprecision of retinol measurements in dried spots was not significantly different from those of the control (plasma). A good correlation coefficient (r2=0.78) was obtained for dried spots¡¯ retinol measurements versus plasma¡¯s retinol analysis (P < 0.01). Paired t-test showed no significant difference between the DBS and retinol methods on a group level. Imprecision of DBS measurement was acceptable, compared to that of the plasma method. The difference be-tween these two methods was not statistically significant on a group level.Conclusion: Application of DBS standard samples, in which a part of the plasma was replaced with the artificial plasma, was shown to be a reliable calibration mean for retinol measurements in DBS samples. Retinol in dried spots was stable for 90 days. Overall, the DBS method provided a precise measurement of retinol, showing results that were comparable with the measurement of retinol in plasma.
%K Vitamin A
%K Retinol
%K Dried Blood Spot
%K HPLC
%K Nutrition
%U http://journals.tbzmed.ac.ir/PDF/HPP/Manuscript/HPP-2-180.pdf