%0 Journal Article
%T Micropropagation of Pluchea lanceolata (Oliver & Hiern.) ¨C A potent anti-arthritic medicinal herb
%A Deepika ARYA
%A Vidya PATNI
%J International Journal of Medicinal and Aromatic Plants
%D 2013
%I 
%X This paper presents an efficient protocol for micropropagation of a medicinally potent anti-arthritic, threatened herb Pluchea lanceolata using shoot tip explants. Shoot tips were cultured on Murashige and Skoog¡¯s (MS) basal medium supplemented with different cytokinins and auxins singly as well as in combinations. Multiple shoot (20.8¡À0.56) proliferation was obtained on MS medium fortified with 0.5 mg/l 6-benzylaminopurine (BAP) and 0.5 mg/l Kinetin (Kn). Elongation and further development of shoot buds into shoots (18.0¡À0.40) was achieved on MS medium fortified with 0.25 mg/l BAP and 0.5 mg/l Kn. Maximum number of roots (16.4¡À0.53) and root length (9.2¡À0.48) was observed in   strength of MS medium supplemented with 3.0 % sucrose and 1.0 mg/l Indole-3-butyric acid (IBA). Plantlets were successfully acclimatized with 70 % survival rate in pots. Thus this study provides a highly efficient, reliable and reproducible protocol for mass multiplication and conservation of Pluchea lanceolata and hence could be helpful to establish and cultivate this threatened medicinal species for its possible role in a wide array of health promoting benefits as anti-oxidant, anti-inflammatory and anti-cancerous agents in the future.
%K Micropropagation
%K MS medium
%K Shoot tip explant
%K Plant growth regulators
%K Pluchea lanceolata (Oliver & Hiern.)
%U http://www.openaccessscience.com/pdf-files/vol3_1_mar2013/IJMAP_3_1_8_Pluchea_lanceolata.pdf