%0 Journal Article
%T In vitro expansion of the mammary stem/progenitor cell population by xanthosine treatment
%A Ratan K. Choudhary
%A Anthony V. Capuco
%J BMC Cell Biology
%D 2012
%I BioMed Central
%R 10.1186/1471-2121-13-14
%X In vitro treatment with xanthosine increased the population of MEC during the exponential phase of cell growth, reducing the doubling time from 86£¿h in control cultures to 60£¿h in xanthosine-treated cultures. The bromodeoxyuridine (BrdU) labeling index and the proportion of MEC in S-phase both were increased by xanthosine treatment, indicating that increased cell accretion was due to increased cell proliferation. Analysis of daughter-pairs indicated that xanthosine promoted a shift from asymmetric to symmetric cell division. Moreover, the 30£¿% increase in symmetric cell division was concomitant with an increase in the proportion of MEC that were positive for a putative stem cell marker (FNDC3B) and a trend toward increased telomerase activity. These results suggest that xanthosine treatment in vitro can increase cell proliferation, promote symmetric cell division and enhance stem/progenitor cell activity.Xanthosine treatment increased the proliferation rate of bovine MEC in vitro. This was likely to be mediated by an increase in the proportion of stem/progenitor cells in the MEC population due to promotion of symmetrical stem cell division by xanthosine.
%K Mammary stem cell
%K Self renewal
%K Symmetric division
%K FNDC3B
%K Telomerase
%K Bovine
%U http://www.biomedcentral.com/1471-2121/13/14/abstract