%0 Journal Article
%T Emulsified Isoflurane Preconditioning Reduces Lung Injury Induced By Hepatic Ischemia/Reperfusion in Rats
%A Xin Lv
%A Zhen-meng Wang
%A Sheng-dong Huang
%A Shao-hua Song
%A Fei-xiang Wu
%A Wei-feng Yu
%J International Journal of Medical Sciences
%D 2011
%I Ivyspring International Publisher
%X Objective: To investigate whether emulsified isoflurane preconditioning could reduce lung injury induced by hepatic I/R in rats and its mechanism. Materials and methods: 32 pentobarbital-anesthetized Sprague-Dawley rats were equally randomized into four groups: laparotomy group (Sham group), hepatic I/R and normal saline infusion group (I/R+S group), I/R and lipid vehicle infusion (I/R+V group), or I/R and 8% emulsified isoflurane infusion (I/R+E group) at the rate of 8 ml¡¤kg-1¡¤h-1 for 30 min. Blood supply of the hepatic artery and portal vein to the left and the median liver lobes was occluded for 90 min after 30-min washout time. Reperfusion was allowed to proceed for 4 h before sacrifice of the animals. Lung injury was observed histologically. Neutrophil infiltration and TNF-¦Á concentration in serum and lung were measured. Changes of wet-to-dry weight ratios in lung tissue, ICAM-1 expression and NF-¦ÊB activity in lung after hepatic I/R were determined. Results: Compared with I/R+S or I/R+V group, emulsified isoflurane preconditioning reduced hepatic I/R-induced lung histologic injury and inhibited the increase of myeloperoxidase (MPO) activity in the lung tissue markedly (5.5¡À1.37 and 5.22¡À1.33 vs 3.81¡À1.62 U/g, P<0.05). In addition, both serum and lung tissue TNF-¦Á levels were reduced in I/R+E group (104.58¡À31.40 and 94.60¡À22.23 vs 72.44¡À17.28 pg/ml, P<0.05; 393.51¡À88.22 and 405.46¡À102.87 vs 292.62¡À74.56 pg/ml, P<0.01). Emulsified isoflurane preconditioning also inhibited the increase of ICAM-1 expression (0.79¡À0.17 and 0.84¡À0.24 vs 0.62¡À0.21, P<0.05) and NF-¦ÊB translocation (4.93¡À0.48 and 4.76¡À0.57 vs 4.01¡À0.86, P<0.05) in the lung tissue markedly. Conclusions: Emulsified isoflurane preconditioning markedly attenuated hepatic I/R-induced lung injury in rats, which may be hopefully applied to the clinical treatment of organ injury caused by hepatic surgery, transplantation or hemorrhagic shock.
%U http://www.medsci.org/v08p0353.htm