%0 Journal Article
%T Correction: RNA interference-mediated gene silencing in murine T cells: in vitro and in vivo validation of proinflammatory target genes
%A Tatjana C Gust
%A Luisa Neubrandt
%A Claudia Merz
%A Khusru Asadullah
%A Ulrich Z¨¹gel
%A Arne von Bonin
%J Cell Communication and Signaling
%D 2008
%I BioMed Central
%R 10.1186/1478-811x-6-3
%X RNAi is a naturally occurring mechanism of gene regulation conserved in plants and mammalian cells. The mechanism of RNAi was identified and characterized almost a decade ago in the nematode worm Caenorhabditis elegans [2]. It relies on double-stranded (ds), small interfering RNAs (siRNAs) of 21¨C23 nucleotides that induce sequence-specific gene silencing in mammalian cells [3]. siRNAs derive from long dsRNAs that trigger the RNAi machinery and are cleaved by the RNaseIII-like enzyme Dicer [4]. siRNAs are incorporated into the RNA-induced silencing complex (RISC) that comprises helicase, exonuclease, and homology-searching domains. The siRNA duplex is unwound and the antisense strand mediates mRNA recognition and thereby, mRNA degradation and subsequent gene silencing. Over the last few years, RNAi has evolved as a new technology for specific gene knockdown that allows analysis of gene function in vitro and in vivo and the identification of new molecular targets associated with disease, such as cancer and inflammation [5-8].T cells constitute one of the major components of the adaptive, cellular immune system. They play a pivotal role in the onset, balance and maintenance of immune responses as well as autoimmunity. Adaptive immune responses are initiated by interactions of T cells with antigen-presenting cells (APC), such as dendritic cells, in the secondary lymphoid organs. T cells become activated and develop into effector cells by a series of activation signals consisting of i) antigen presentation by APC via peptide-major histocompatibility (MHC) complexes that stimulate the T-cell receptor (TCR, [9], ii) costimulation by B7 family members that bind to CD28 expressed on T cells [10]. Upon engagement of the TCR, a signaling cascade inside the cell is initiated that leads to activation of the T cell. The Src family of protein tyrosine kinases Fyn and Lck become activated and phosphorylate the cytoplasmic domains of the CD3 complex which then interact with the zeta
%U http://www.biosignaling.com/content/6/1/3