%0 Journal Article
%T Genotipificaci¨®n del Virus del Papiloma Humano(VPH) en muestras de cepillados cervicales de pacientes de diferentes hospitales de Bogot¨¢ y evaluaci¨®n de la concordancia de dos m¨¦todos basados en PCR Genotyping the human papilloma virus (HPV) in Cytobrush samples taken from patients attending different hospitals in Bogot¨¢ (Colombia) and evaluating agreement between two PCR-based methods
%A Dabeiba A Garc¨ªa
%A Markus Schmitt
%A ¨¢ngel Cid-Arregui
%A Marcos Castillo
%J Revista Colombiana de Obstetricia y Ginecolog¨ªa
%D 2010
%I Federaci¨®n Colombiana de Asociaciones de Obstetricia y Ginecolog¨ªa
%X Objetivo: genotipificar el VPH en muestras de pacientes con diferentes grados histopatol¨®gicos de lesi¨®n cervical. Materiales y m¨¦todos: estudio de corte transversal en muestras de cepillado cervical de mujeres con diferentes grados de lesiones cervicales histopatol¨®gicas en la ciudad de Bogot¨¢ en el a o 2009. Se utiliz¨® Luminex  xMAP  una t¨¦cnica altamente sensible, reproducible y automatizada, y Reverse Line Blot (RLB). Resultados: las infecciones por los tipos de VPH de 16 y 18 fueron las m¨¢s frecuentes en todos los tipos de patolog¨ªa del c¨¦rvix. Las infecciones mixtas predominaron con un 78,04% de los casos con patolog¨ªa negativa, 85,7% NIC I, 81,25% NIC II, 78,78% NIC III y 71,42% c¨¢ncer cervical. La concordancia entre los dos m¨¦todos fue del 67%. Conclusi¨®n: las infecciones por los tipos de VPH 16 y 18 predominan en la poblaci¨®n estudiada. Objective: genotyping HPV in samples taken from patients having different histopathological degrees of cervical injury. Materials and methods: this was a cross-sectional study of cervical scrape samples taken from females having different degrees of histopathological cervical injury in Bogot¨¢ during 2009. Luminex  xMAP  was used, which is a highly sensitive, reproducible and automated technique, as well as reverse line blot (RLB). Results: infections caused by HPV types 16 and 18 were the most frequently found types of pathology of the cervix. Mixed infections predominated (78.04%); amongst cases having negative pathology, 85.7% was found for cervical intraepithelial neoplasia (CIN) I, 81.25% for CIN II, 78.78% for CIN III and 71.42% for cervical cancer. Agreement between both methods was 67%. Conclusion: infection by VPH types 16 and 18 predominated in the population being studied.
%K papilomavirus
%K displasia cervical
%K patolog¨ªa
%K biolog¨ªa molecular
%K papilloma virus
%K cervical dysplasia
%K pathology
%K molecular biology
%U http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0034-74342010000400004