%0 Journal Article
%T Role of tissue factor pathway inhibitor-2 in the expressions of matrix metallopro- teinases in keratocytes in vitro
%A Jing Yuan
%A Jian-Xiong Yu
%A Lian-Hong Zhou
%J Guoji Yanke Zazhi
%D 2013
%I IJO Press
%R 10.3980/j.issn.1672-5123.2013.04.03
%X AIM:To elucidate the relation between tissue factor pathway inhibitor-2(TFPI-2)expression and the expression of matrix metalloproteinases(MMPs)in keratocytes. METHODS: Primary culture and subculture of rabbit keratocytes were established in vitro. Plasmid vector pBos-Cite-neo/TFPI-2 was transfected into keratocytes with Lipofectamine 2000. After being selected by G418, three groups of cells including TFPI-2 gene transfected cells K-TFPI-2, empty vector transfected cells K-V and non-transfected cells K-P were screened for TFPI-2 mRNA and protein by reverse transcription-polymerase chain reaction and Western blot analysis, respectively. The activity of MMPs in the three groups of cells was detected by substrate zymography and compared by ANOVA. RESULTS: Expression of mRNA and protein of TFPI-2 was more in the cells of K-TFPI-2 than in the other cells of K-P and K-V with a significant difference(mRNA:0.79¡À0.02 vs 0.51¡À0.03 and 0.48¡À0.02, P=0.000 and P=0.000; Protein:24.5¡À0.8 vs 15.5¡À0.5 and 14.9¡À0.9,P=0.000 and P=0.000). Compared with the two groups of K-P and K-V, the cells of K-TFPI-2 had a significant decreased activity of MMP1(12.3¡À0.7 vs 16.7¡À1.2 and 15.9¡À0.7, P=0.001 and P=0.003)and MMP2(15.4¡À1.3 vs 18.2¡À1.1 and 17.8¡À1.1, P=0.027 and P=0.046). CONCLUSION: It is suggested that the expression of TFPI-2 may strongly inhibit the activity of MMPs in keratocytes in vitro, which provides an experimental basis for curing CNV with gene therapy.
%K keratocytes
%K tissue factor pathway inhibitor-2
%K matrix metalloproteinases
%K extracellular matrix
%U http://www.ies.net.cn/cn_publish/2013/4/201304003.pdf