%0 Journal Article
%T Selecci車n de hongos entomopat車genos para controlar salivazos (Hemiptera: Cercopidae) de la ca a de az迆car en Colombia Selection of entomopathogenic fungi to control spittlebugs (Hemiptera: Cercopidae) of sugar cane in Colombia
%A ANAMAR赤A GARC赤A D
%A ALEX ENRIQUE BUSTILLO P
%A ULISES CASTRO V
%A YOLIMA ARENAS B
%J Revista Colombiana de Entomolog赤a
%D 2012
%I Sociedad Colombiana de Entomolog赤a
%X Los salivazos (Hemiptera: Cercopidae) amenazan con convertirse en plagas de la ca a de az迆car en Colombia. Desde el 2007, se detect車 Aeneolamia varia en la zona central del Valle del Cauca, lo cual motiv車 a los productores de ca a de az迆car a tomar medidas de emergencia para controlar estas infestaciones. Otra especie Zulia carbonaria tambi谷n se registra atacando ca a de az迆car y se us車 en este estudio debido a restricciones cuarentenarias impuestas a A. varia. Se usaron larvas de Galleria mellonella como trampa en el suelo, para obtener los hongos entomopat車genos. Los aislamientos se reactivaron sobre Z. carbonaria y se caracterizaron en relaci車n con su tasa de crecimiento, esporulaci車n, germinaci車n y patogenicidad. Se obtuvieron 26 hongos entomopat車genos (15 aislamientos de Metarhizium anisopliae y 11 de Paecilomyces lilacinus). Solo ocho aislamientos de M. anisopliae y seis de P. lilacinus resultaron patog谷nicos al salivazo. Las cepas de M. anisopliae CCMa0907 y de P. lilacinus: CCPL0801, CCPL0904, CCPL0802 y CCPL0805, presentaron caracter赤sticas ideales de esporulaci車n, tasa de crecimiento y porcentaje de germinaci車n. Finalmente, se desarroll車 el protocolo para realizar bioensayos en invernadero, para evaluar el potencial patog谷nico de hongos para el control de salivazos de la ca a de az迆car. Spittlebugs (Hemiptera: Cercopidae) can become into a menace to the sugar cane pest in Colombia. Aeneolamia varia was detected in 2007 in the central area of Cauca Valley, which made sugar cane producers to take emergency measures to control this pest. Another species Zulia carbonaria is also present infesting sugar cane, but due to A. varia quarantine restrictions, Z. carbonaria was used in this research. Galleria mellonella larvae were used as a bait to obtain entomopathogenic fungi from soil samples. Fungi isolates were reactivated using adults of Z. carbonaria and characterized by measuring the speed of development, sporulation, viability and pathogenicity. Twenty-six entomopathogenic fungi were obtained 15 strains of Metarhizium anisopliae and 11 strains of Paecilomyces lilacinus, but only eight strains of M. anisopliae and six of P. lilacinus, were pathogenic to adults of Z. carbonaria. The isolates of M. anisopliae: CCMa0907 and P. lilacinus: CCPL0801, CCPL0904, CCPL0802, CCPL0805, had the best characteristics of growth, viability and sporulation. Finally, a protocol to conduct a bioassay was developed, under greenhouse conditions, to test strains of pathogenic fungi to control sugarcane spittlebugs.
%K Zulia carbonaria
%K Aeneolamia varia
%K Metarhizium anisopliae
%K Paecilomyces lilacinus
%K Zulia carbonaria
%K Aeneolamia varia
%K Metarhizium anisopliae
%K Paecilomyces lilacinus
%U http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-04882012000200015