%0 Journal Article
%T Influence of the extraction procedure on the antioxidative activity of lentil seed extracts in a  2-carotene-linoleate model system
%A Amarowicz
%A R.
%A Karamac
%A M.
%A Chavan
%A U.
%J Grasas y Aceites
%D 2001
%I Consejo Superior de Investigaciones Cient赤ficas
%X Phenolic compounds were extracted from lentil seeds using three solvent systems: 80% (v/v) acetone, 80% (v/v) methanol, and 80% (v/v) ethanol. Each extract was subsequently separated into two fractions by chromatoghraphy on a column with Toyo Pearl HW-40 using water (fraction I) and methanol (fraction II) for elution. Antioxidative activity of extracts and their respective fractions were examined in a  2-carotene-linoleate model system. All three extracts exhibited similar antioxidant activity. Considering the level of phenolic compounds in extracts it seems that phenolic compounds from the acetone extract were less active than those from either the methanolic and ethanolic ones. Because the content of phenolics was about 16-fold lower in fraction I of the methanolic and ethanolic extracts compared to fraction II, the phenolics in fraction I of the methanol and ethanol extracts from lentil seeds are much more active than these in fraction II. A stronger antioxidant activity of fraction I from the acetone extract compared to the crude acetone extract was observed during the latter incubation stage. The reason was a relatively high level of phenolic compounds in this fraction. UV spectra confirmed that the phenolic compounds from the acetone extract were different compared to methanolic and ethanolic extracts. Los compuestos fen車licos fueron extra赤dos de semillas de lenteja usando tres sistemas de disolventes: acetona del 80% (v/v), metanol del 80% (v/v), y etanol del 80% (v/v). Cada extracto fue separado posteriormente en dos fracciones por cromatograf赤a en columna con Toyo Pearl HW-40 usando agua (fracci車n I) y metanol (fracci車n II) para la eluci車n. La actividad antioxidante de los extractos y de sus fracciones respectivas fueron examinadas en un sistema modelo  2-caroteno-linoleato. Los tres extractos exhibieron actividad antioxidante similar. Atendiendo al nivel de compuestos fen車licos en los extractos parece que los compuestos del extracto acet車nico eran menos activos que los metan車licos y etan車licos. Debido a que el contenido de fenoles era aproximadamente 16 veces m芍s bajo en la fracci車n I de los extractos metan車lico y etan車lico comparado con la fracci車n II, los fenoles en la fracci車n I de los extractos del metanol y del etanol de las semillas de lenteja son mucho m芍s activos que 谷stos en la fracci車n II. Una actividad antioxidante m芍s fuerte de la fracci車n I del extracto acet車nico comparado con el extracto acet車nico crudo fue observada durante el posterior periodo de incubaci車n. La raz車n fu谷 el relativamente alto nivel de compuestos fen車licos en es
%K Antioxidative activity
%K 
%K 2
%K -carotene linoleate
%K Extraction
%K Lentil seed (extract)
%K Actividad antioxidante
%K 2-caroteno linoleato
%K Extracci車n
%K Semilla de lenteja (extracto)
%U http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/378/381