%0 Journal Article
%T Interstitial fluid pressure, vascularity and metastasis in ectopic, orthotopic and spontaneous tumours
%A Sarah Lunt
%A Tuula MK Kalliomaki
%A Allison Brown
%A Victor X Yang
%A Michael Milosevic
%A Richard P Hill
%J BMC Cancer
%D 2008
%I BioMed Central
%R 10.1186/1471-2407-8-2
%X KHT-C murine fibrosarcoma, ME180 and SiHa human cervix carcinoma were grown either intramuscularly (i/m), sub-cutaneously (s/c) or orthotopically. Polyoma middle-T (MMTV-PyMT) transgenic spontaneous mammary tumours were studied either as spontaneous tumours or following orthotopic or i/m transplantation. IFP was measured in all tumours using the wick-in-needle method. Spontaneous metastasis formation in the lungs or lymph nodes was assessed in all models. An immunohistochemical analysis of tumour hypoxia, vascular density, lymphatic vascular density and proliferation was carried out in ME180 tumours grown both i/m and orthotopically. Blood flow was also assessed in the ME180 model using high-frequency micro-ultrasound functional imaging.Tumour IFP was heterogeneous in all the models irrespective of growth site: KHT-C i/m: 2每42 mmHg, s/c: 1每14 mmHg, ME180: i/m 5每68 mmHg, cervix 4每21 mmHg, SiHa: i/m 20每56 mmHg, cervix 2每26 mmHg, MMTV-PyMT: i/m: 13每45 mmHg, spontaneous 2每20 mmHg and transplanted 2每22 mmHg. Additionally, there was significant variation between individual tumours growing in the same mouse, and there was no correlation between donor and recipient tumour IFP values. Metastatic dissemination to the lungs or lymph nodes demonstrated no correlation with tumour IFP. Tumour hypoxia, proliferation, and lymphatic or blood vessel density also showed no relationship with tumour IFP. Speckle variance analysis of ultrasound images showed no differences in vascular perfusion between ME180 tumours grown i/m versus orthotopically despite differences in IFP.Our studies across a range of different tumour models showed substantial heterogeneity in tumour IFP, suggesting differences in the vascular development and interstitial fluid dynamics in the individual tumours. The results demonstrate a strong stochastic aspect to tumour IFP development, notably the variation apparent between different tumours within the same animal and the lack of correlation between donor and recip
%U http://www.biomedcentral.com/1471-2407/8/2