%0 Journal Article
%T Allelic frequency of the Kappa-Casein gene in Colombian and creole cattle breeds Frecuencia al¨¦lica del gen Kappa-Case¨ªna en bovinos criollos y colombianos Freq¨¹¨ºncia do alelo de gene de Kappa-Case¨ªna em bovinos crioulos e colombianos
%A Jaime A Rosero
%A Luz A ¨¢lvarez
%A Jaime E Mu£¿oz
%A Carlos V Dur¨¢n
%J Revista Colombiana de Ciencias Pecuarias
%D 2012
%I Universidad de Antioquia
%X Colombia has one of the most genetically diverse creole cattle populations, with eight creole breeds and two improved creole (Colombian) breeds. A high demand for meat and milk has led to the inevitable selection of highly productive cattle and the introduction of foreign breeds. Unfortunately, these breeds are often ill-suited for tropical conditions. These factors threaten the size of the creole livestock population, which is considered part of Colombia's national heritage. Objective: to estimate the allelic frequencies of the Kappa-Casein gene (CNS3) in Colombian creole cattle breeds (GCC). Methods: a total of 354 blood samples were taken from 30 animals of each of the following breeds: Blanco Orejinegro (BON), Caquete o (CQT), Casanare o (CAS), Horned Coste o (Coste o con Cuernos, CCC), Chino Santandereano (ChS), Hart¨®n del Valle (HV), Romosinuano (ROM), and Sanmartinero (SM), each representing the 8 established ''criollo'' (creole) breeds; the Lucerna (LUC) and Velasquez (VEL) representing the two Colombian improved breeds; and Brahman and Holstein as control breeds. DNA was extracted by a salting-out procedure and a 453 bp fragment on chromosome 6 was amplified by PCR. CSN3 alleles were identified using single strand conformation polymorphism (SSCP) and their sequence compared with those of the Genebank for Bos taurus and Bos indicus. Results: higher frequencies for allele variants of CSN3 A (0.39) and B (0.41) were found relative to the frequencies of I (0.038), G (0.095), A1 (0.025), E (0.006), and N (0.006). The allele of interest (CSN3 B) had a high frequency in the CCC (0.81), ROMO (0.66), CQT (0.55), ChS (0.48), and VEL (0.43) breeds. Conclusions: these findings suggest that Colombian creole breeds harbor a high genetic diversity which enriches its gene pool and warrants future conservation efforts to protect its integrity. Colombia es uno de los pa¨ªses m¨¢s diversos en recursos gen¨¦ticos criollos. Posee ochos razas de ganado criollo (GCC) y dos razas de criollo mejorado o razas colombianas. La creciente demanda de alimentos ha generado una forzosa selecci¨®n de individuos altamente productivos e introducci¨®n de razas for¨¢neas (Holstein y Brahman) poco adaptadas a condiciones tropicales, lo que ha puesto en riesgo el tama o efectivo del ganado criollo, considerado patrimonio nacional. Objetivo: estimar la frecuencia al¨¦lica del gen (CNS3) de la Kappa-Case¨ªna en el (GCC). M¨¦todos: se usaron 354 muestras de sangre de ocho razas bovinas criollas (30 individuos por raza): Blanco Orejinegro (BON), Caquete o (CQT), Casanare o (CAS), Coste o con Cuern
%K marcadores moleculares
%K PCR-SSCP
%K prote¨ªnas leche
%K variantes gen¨¦ticas
%K marcadores moleculares
%K PCR-SSCP
%K prote¨ªnas do leite
%K variantes gen¨¦ticas
%K genetic variants
%K milk proteins
%K molecular markers
%K PCR-SSCP
%U http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902012000200002