%0 Journal Article
%T The possible role of ribosomal protein S6 kinase 4 in the senescence of endothelial progenitor cells in diabetes mellitus
%A Zhiyong Yin
%A Linni Fan
%A Gaosheng Huang
%A Haichang Wang
%A Zhe Wang
%J Cardiovascular Diabetology
%D 2012
%I BioMed Central
%R 10.1186/1475-2840-11-12
%X These above lead to consideration of an evidence-based hypothesis that RSK4 may serve as a mediator of EPCs senescence in DM.EPCs of healthy subjects and DM patients are isolated from peripheral blood and incubated with high glucose (HG). Then, the EPCs senescence would be detected by senescence associated ¦Â-galactosides (SA-¦Â-gal) staining. Meanwhile, the RSK4 expression is assessed by RT-PCR and western blot. Moreover, overexpressing or RNA interfering of RSK4 in EPCs to investigate the relationship between RSK4 expression and the senescence of EPCs are necessary to substantiate this hypothesis. Also, studies on possible upstream and downstream factors of RSK4 would be explored to reveal the RSK4-mediated senescence pathway in EPCs.If proved, this hypothesis will provide another mediator of EPCs senescence, and may establish a novel pathogenesis for DM and further benefit to the management of DM.EPCs are first reported in 1997 [1], which are derived from the bone marrow and could be mobilized to the peripheral circulation in response to stimuli. EPCs have been believed to be angioblasts and contribute to neovascularization, vascular maintenance and repair in adults, and EPCs dysfunction may enhance the risk for cardiovascular disease, DM and tumor [2,3]. Emerging evidence has showed the count and function of EPCs are impaired in DM [4-6]. Moreover, diabetes could alter the subpopulation of EPCs by impairing the production in the bone marrow and decreasing the mobilization from the spleen [7]. Likewise, Jung C et. al found that DM patients had a smaller number of CD34-/CD133+ EPCs, but a larger proportion of apoptotic EPCs [8]. Besides, the reduction of EPCs may augment with an increased number of diseased coronary arteries, which may aggravate the DM and the complications [9]. And it is proved that increased EPCs number could promote the revascularization in asymptomatic type 2 diabetic patients [10,11].The mechanisms of the EPCs impairment are largely unknown. Re
%K Diabetes mellitus
%K Endothelial progenitor cell
%K RSK4
%K Senescence
%U http://www.cardiab.com/content/11/1/12