%0 Journal Article
%T Expression of fourteen novel obesity-related genes in zucker diabetic fatty rats
%A Peter M Schmid
%A Iris Heid
%A Christa Buechler
%A Andreas Steege
%A Markus Resch
%A Christoph Birner
%A Dierk H Endemann
%A Guenter A Riegger
%A Andreas Luchner
%J Cardiovascular Diabetology
%D 2012
%I BioMed Central
%R 10.1186/1475-2840-11-48
%X We performed an expression analysis of fourteen genes, namely BDNF, ETV5, FAIM2, FTO, GNPDA2, KCTD15, LYPLAL1, MCR4, MTCH2, NEGR1, NRXN3, TMEM18, SEC16B and TFAP2B, via real-time RT-PCR in adipose tissue of the kidney capsule, the mesenterium and subcutaneum as well as the hypothalamus of obese Zucker diabetic fatty (ZDF) and Zucker lean (ZL) rats at an age of 22£¿weeks.All of our target genes except for SEC16B showed the highest expression in the hypothalamus. This suggests a critical role of these obesity-related genes in the central regulation of energy balance. Interestingly, the expression pattern in the hypothalamus showed no differences between obese ZDF and lean ZL rats. However, LYPLAL1, TFAP2B, SEC16B and FAIM2 were significantly lower expressed in the kidney fat of ZDF than ZL rats. NEGR1 was even lower expressed in subcutaneous and mesenterial fat, while MTCH2 was higher expressed in the subcutaneous and mesenterial fat of ZDF rats.The expression pattern of the investigated obesity genes implies for most of them a role in the central regulation of energy balance, but for some also a role in the adipose tissue itself. For the development of the ZDF phenotype peripheral rather than central mechanisms of the investigated genes seem to be relevant.By now, overweight and obesity have become a major public health problem in the industrialized world and also in developing countries. Both result in an increased morbidity and mortality, not to mention the financial costs for the health-care systems. According to the World Health Organisation (WHO) in 2005 worldwide at least 400 million adults were obese and this count is expected to rise to 700 million until 2015 (http://www.who.int/gho/ncd/risk_factors/overweight/en/ webcite). Thereby, lifestyle changes with an increased intake of energy-dense foods and a trend to decreased physical activity are fundamental causes for this rise to epidemic proportions, but heritability studies provide evidence for a notable genet
%K BDNF
%K ETV5
%K FAIM2
%K FTO
%K GNPDA2
%K KCTD15
%K LYPLAL1
%K MCR4
%K MTCH2
%K NEGR1
%K NRXN3
%K TMEM18
%K SEC16B
%K TFAP2B
%K ZDF-rats
%U http://www.cardiab.com/content/11/1/48