%0 Journal Article
%T Effect of vitamin D on aortic remodeling in streptozotocin-induced diabetes
%A Erik Salum
%A Priit Kampus
%A Mihkel Zilmer
%A Jaan Eha
%A Mark Butlin
%A Alberto P Avolio
%A Taavi P£¿dram£¿gi
%A Andres Arend
%A Marina Aunapuu
%A Jaak Kals
%J Cardiovascular Diabetology
%D 2012
%I BioMed Central
%R 10.1186/1475-2840-11-58
%X Male Wistar rats (n£¿=£¿30) were randomly assigned to control untreated, diabetic untreated, and diabetic£¿+£¿cholecalciferol groups. Diabetes was induced by intraperitoneal injection of streptozotocin, followed by oral administration of cholecalciferol (500£¿IU/kg) for 10£¿weeks in the treatment group. Aortic pulse wave velocity (PWV) was recorded over a mean arterial pressure (MAP) range of 50 to 200£¿mmHg using a dual pressure sensor catheter. Intravenous infusion of phenylephrine and nitroglycerine was used to increase and decrease MAP, respectively. Serum 25-hydroxyvitamin D [25(OH)D] levels were measured using a radioimmune assay. ADMA levels in serum were measured by enzyme-linked immunoassay. Aortic samples were collected for histomorphometrical analysis.PWV up to MAP 170£¿mmHg did not reveal any significant differences between all groups, but in diabetic rats, PWV was significantly elevated across MAP range between 170 and 200£¿mmHg. Isobaric PWV was similar between the treated and untreated diabetic groups, despite significant differences in the levels of serum 25(OH)D (493£¿¡À£¿125£¿nmol/L vs 108£¿¡À£¿38£¿nmol/L, respectively). Serum levels of ADMA were similarly increased in the treated and untreated diabetic groups, compared to the control group. The concentration and integrity of the elastic lamellae in the medial layer of the aorta was impaired in untreated diabetic rats and improved by vitamin D supplementation.PWV profile determined under isobaric conditions demonstrated differential effects of uncontrolled diabetes on aortic stiffness. Diabetes was also associated with elevated serum levels of ADMA. Vitamin D supplementation did not improve the functional indices of aortic stiffness or endothelial function, but prevented the fragmentation of elastic fibers in the aortic media.Cardiovascular (CV) events are considered to be a principal cause of mortality in patients with diabetes mellitus (DM) [1]. Macrovascular complications of DM are associated with stiffening of
%K Streptozotocin
%K Aortic stiffness
%K Pulse wave velocity
%K Elastin
%K Asymmetric dimethylarginine
%K Vitamin D
%U http://www.cardiab.com/content/11/1/58