%0 Journal Article
%T Flow-cytometric monitoring of disease-associated expression of 9-O-acetylated sialoglycoproteins in combination with known CD antigens, as an index for MRD in children with acute lymphoblastic leukaemia: a two-year longitudinal follow-up study
%A Suchandra Chowdhury
%A Suman Bandyopadhyay
%A Chandan Mandal
%A Sarmila Chandra
%A Chitra Mandal
%J BMC Cancer
%D 2008
%I BioMed Central
%R 10.1186/1471-2407-8-40
%X A two-year longitudinal follow-up study was done with 109 patients from the onset of the disease till the end of chemotherapy, treated under MCP841protocol. Paired samples of PB (n = 1667) and BM (n = 999) were monitored by flow cytometry. Three templates selected for this investigation were OAcSGP+CD10+CD19+ or OAcSGP+CD34+CD19+ for B-ALL and OAcSGP+CD7+CD3+ for T-ALL.Using each template the level of MRD detection reached 0.01% for a patient in clinical remission (CR). 81.65% of the patients were in CR during these two years while the remaining relapsed. Failure in early clearance of lymphoblasts, as indicated by higher MRD, implied an elevated risk of relapse. Soaring MRD during the chemotherapeutic regimen predicted clinical relapse, at least a month before medical manifestation. Irrespective of B- or T-lineage ALL, the MRD in PB and BM correlated well.A range of MRD values can be predicted for the patients in CR, irrespective of their lineage, being 0.03 ¡À 0.01% (PB) and 0.05 ¡À 0.015% (BM). These patients may not be stated as normal with respect to the presence of MRD. Hence, MRD study beyond two-years follow-up is necessary to investigate further reduction in MRD, thereby ensuring their disease-free survival. Therefore, we suggest use of these templates for MRD detection, during and post-chemotherapy for proper patient management strategies, thereby helping in personalizing the treatment.Acute lymphoblastic leukaemia (ALL) is a malignant transformation of lymphoblasts, representing the single commonest type of cancer in paediatric population. With the dawn of modern chemotherapy, virtually all children attain remission and approximately 80% are cured, but the risk of relapse remains as about 20% patients in clinical remission (CR) harbour residual leukemic blasts referred to as minimal residual disease (MRD) [1]. The situation is however dissimilar in India. Data from various Indian cancer registries suggest occurrence of approximately 10,000 new cases of child
%U http://www.biomedcentral.com/1471-2407/8/40