%0 Journal Article
%T Presencia del elemento gen谷tico transponible dTdic1 en Dianthus caryophyllus con flores variegadas y no variegadas
%A L車pez Castro Karen Roc赤o
%A Franco-Lara Liliana
%J Revista Colombiana de Biotecnolog赤a
%D 2012
%I Universidad Nacional de Colombia
%X T赤tulo en ingl谷s: Presence of the transposable genetic element  dTdic1 in Dianthus caryophyllus with  variegated and no variegated flowers Resumen: El objetivo de este trabajo era la b迆squeda del  EGT (Elemento Gen谷tico Transponible) dTdic1 que ha sido asociado con la variegaci車n del color de las flores de clavel y su relaci車n con dos genes que codifican para enzimas involucradas en la bios赤ntesis de antocianinas,  Chalcona isomerasa (CHI) y Dihidroflavonol reductasa (DFR).  Su presencia y expresi車n se evalu車 en siete genotipos con flores variegadas (l赤neas h赤bridas) y en cuatro genotipos de flores no variegadas (una l赤nea h赤brida y tres cultivares comerciales).  Un alto n迆mero (indefinido) de copias del elemento dTdic1 se detect車 en todas las l赤neas variegadas y no variegadas.  En consecuencia, la sola presencia de este EGT no pudo asociarse directamente con la variegaci車n de los pigmentos florales de flores de clavel.  Adicionalmente, dTdic1 se encontr車 interrumpiendo el gen CHI en cuatro genotipos variegados y uno no variegado. No se observ車 evidencia de inserci車n de dTdic1 en el gen DFR en ninguno de los genotipos. Palabras clave: Transposones; chalcona isomerasa; antocianinas Abstract: The objective of this work was to search for the EGT (Transposable Genetic Element) dTdic1 that has been associated with color variegation of carnation flowers and its relationship with two genes that code for enzymes involved in the synthesis of anthocyanins, Chalcona isomerase (CHI) and Dihidroflavonol reductase (DFR). Its presence and expression was evaluated in seven genotypes of variegated flowers and four no variegated flower genotypes (one hybrid line and three commercial cultivars). A high number of copies (undefined) of copies of the dTdic1 element was detected in variegaated and no variegated lines. Therefore, the main presence of this EGT was not associated directly with variegation of floral pigments. Additionally, dTdic1 was found interrupting the CHI gene in four variegated and one no variegated phenotypes. No evidence was observed of insertion of dTdic1 in the DFR gene in any of the genotypes. Key words: Transposons; chalcone isomerase; anthocyanins.
%K Transposones
%K chalcona isomerasa
%K antocianinas
%K Transposons
%K chalcone isomerase
%K anthocyanins
%U http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/37246