%0 Journal Article
%T Is an alcoholic fixative fluid used for manual liquid-based cytology accurate to perform HPV tests?
%A Garbar C
%A Mascaux C
%A De Graeve P
%A Delvenne P
%J Pathology and Laboratory Medicine International
%D 2011
%I Dove Medical Press
%X Christian Garbar1, Corinne Mascaux1, Philippe De Graeve2, Philippe Delvenne31Department of Biopathology, Institute Jean Godinot, Reims Cedex, France; 2Centre de Pathologie des Coteaux, Toulouse, France; 3Department of Pathology, University of Liege, Tour de Pathologie, Domaine Universitaire du Sart Tilman, Li¨¨ge, BelgiumAbstract: In Europe, the alternative centrifuge method of liquid-based cytology is widely used in cervical screening. Turbitec  (Labonord SAS, Templemars, France) is a centrifuge method of liquid-based cytology using an alcoholic fixative fluid, Easyfix  (Labonord). It is now well accepted that the association of liquid-based cytology and human papillomavirus test is indissociable of cervical screening. The aim of this work was to demonstrate that Easyfix alcoholic fluid is reliable to perform Hybrid Capture  2 (QIAGEN SAS, Courtaboeuf, France). In this study, 75 patients with colposcopy for cervical lesions served as gold standard. A sample was collected, at random, for Easyfix fixative cytological fluid and for Digene Cervical Sampler (QIAGEN). The results of Hybrid Capture 2 (with relative light unit >1) showed no statistical difference, a positive SpearmanĄ¯s correlation (r = 0.82, P < 0.0001), and a kappa value of 0.87 (excellent agreement) between the two fluids. It was concluded that Easyfix is accurate to use in human papillomavirus tests with Hybrid Capture 2.Keywords: human papillomavirus, hybrid capture 2, Turbitec , cervix cytology, liquid-based cytology
%U http://www.dovepress.com/is-an-alcoholic-fixative-fluid-used-for-manual-liquid-based-cytology-a-a8955