%0 Journal Article
%T CD133, CD15/SSEA-1, CD34 or side populations do not resume tumor-initiating properties of long-term cultured cancer stem cells from human malignant glio-neuronal tumors
%A Cristina Patru
%A Luciana Romao
%A Pascale Varlet
%A Laure Coulombel
%A Eric Raponi
%A Josette Cadusseau
%A Fran£¿ois Renault-Mihara
%A C¨¦cile Thirant
%A Nadine Leonard
%A Alain Berhneim
%A Maria Mihalescu-Maingot
%A Jacques Haiech
%A Ivan Bi¨¨che
%A Vivaldo Moura-Neto
%A Catherine Daumas-Duport
%A Marie-Pierre Junier
%A Herv¨¦ Chneiweiss
%J BMC Cancer
%D 2010
%I BioMed Central
%R 10.1186/1471-2407-10-66
%X We screened for TICs in 47 human adult brain malignant tumors. Cells forming floating spheres in culture, and endowed with all of the features expected from tumor cells with stem-like properties were obtained from glioblastomas, medulloblastoma but not oligodendrogliomas.A long-term self-renewal capacity was particularly observed for cells of malignant glio-neuronal tumors (MGNTs). Cell sorting, karyotyping and proteomic analysis demonstrated cell stability throughout prolonged passages. Xenografts of fewer than 500 cells in Nude mouse brains induced a progressively growing tumor. CD133, CD15/LeX/Ssea-1, CD34 expressions, or exclusion of Hoechst dye occurred in subsets of cells forming spheres, but was not predictive of their capacity to form secondary spheres or tumors, or to resist high doses of temozolomide.Our results further highlight the specificity of a subset of high-grade gliomas, MGNT. TICs derived from these tumors represent a new tool to screen for innovative therapies.Tumor initiating cells (TICs) from various types of cancers have been isolated and characterized. The tumors of origin range from glioblastomas and medulloblastomas [1-6] to epithelial tumors of the breast [7], lung [8], colon [9], and prostate [10]. Gliomas represent the majority of primary tumors from the central nervous system (CNS) [11]. Difficulties in clinical management (e.g. treatment and prognosis) are related to the complex identity of gliomas, which lack reliable morphological and molecular signatures, precluding thus the establishment of a clear cut classification discriminating between different tumor subtypes [12].Historically, it has been proposed that gliomas (astrocytomas and oligodendrogliomas) originate respectively from mature astrocytes or oligodendrocytes. The fact that these brain tumors frequently include a mixture of cells expressing neuronal and glial markers, has recently led to the alternative proposal that gliomas arise from neural stem/progenitor cells. Suppor
%U http://www.biomedcentral.com/1471-2407/10/66