%0 Journal Article
%T Microarray analysis of DNA damage repair gene expression profiles in cervical cancer cells radioresistant to 252Cf neutron and X-rays
%A Yi Qing
%A Xue-Qin Yang
%A Zhao-Yang Zhong
%A Xin Lei
%A Jia-Yin Xie
%A Meng-Xia Li
%A De-Bing Xiang
%A Zeng-Peng Li
%A Zhen-Zhou Yang
%A Ge Wang
%A Dong Wang
%J BMC Cancer
%D 2010
%I BioMed Central
%R 10.1186/1471-2407-10-71
%X HeLa cells were treated with fractionated 252Cf neutron and X-rays, with a cumulative dose of 75 Gy each, over 8 months, yielding the sub-lines HeLaNR and HeLaXR. Radioresistant characteristics were detected by clone formation assay, ultrastructural observations, cell doubling time, cell cycle distribution, and apoptosis assay. Gene expression patterns of the radioresistant sub-lines were studied through microarray analysis and verified by Western blotting and real-time PCR.The radioresistant sub-lines HeLaNR and HeLaXR were more radioresisitant to 252Cf neutron and X-rays than parental HeLa cells by detecting their radioresistant characteristics, respectively. Compared to HeLa cells, the expression of 24 genes was significantly altered by at least 2-fold in HeLaNR cells. Of these, 19 genes were up-regulated and 5 down-regulated. In HeLaXR cells, 41 genes were significantly altered by at least 2-fold; 38 genes were up-regulated and 3 down-regulated.Chronic exposure of cells to ionizing radiation induces adaptive responses that enhance tolerance of ionizing radiation and allow investigations of cellular radioresistance mechanisms. The insights gained into the molecular mechanisms activated by these "radioresistance" genes will lead to new therapeutic targets for cervical cancer.Cervical cancer is a worldwide disease, with a high incidence and mortality [1] especially in developing countries. Approximately 500,000 cases of cervical cancer are diagnosed annually, with mortality around 40% [2]. Radiotherapy is particularly effective for patients with cervical cancers at an advanced stage or that cannot be cured surgically. Low LET radiation (e.g., gamma rays, X-rays) is generally used to treat cervical cancers, but its ability to cure local disease decreases with increasing tumor size, because the doses required to treat large tumors exceed the toxicity limits of normal tissues [3]. High LET radiation such as neutron rays is associated with a low rate of repair of poten
%U http://www.biomedcentral.com/1471-2407/10/71