%0 Journal Article
%T Utilizaci車n de la autorradiograf赤a en la detecci車n de ant赤genos predominantes de Trichinella spiralis en tres modelos experimentales WESTERN BLOT AUTORADIOGRAPHY IN THE DETECTION OF DOMINANT ANTIGENS OF Trichinella spiralis IN THREE EXPERIMENTAL ANIMALS MODELS
%A MARIA ALEJANDRA MORENO CARCIA
%A MARIA PATROCINIO RAMOS DE LEON
%A JESUS MUˋOZ ESCOBEDO
%J Parasitolog赤a al d赤a
%D 2001
%I Scientific Electronic Library Online
%X En el presente estudio se llev車 a cabo la detecci車n de ant赤genos predominantes de Trichinella spiralis en 3 modelos experimentales (Rata, rat車n y conejo), mediante Western blot (WB) autorradiograf赤a. A los animales experimentales se les administr車 larvas infectantes del par芍sito y se sangraron pre-inoculaci車n y a la cuarta semana postinfecci車n. El AST (Ant赤geno soluble de Trichinella) se obtuvo a partir de larvas de m迆sculo de rata experimentalmente infectadas. Con el AST se realizaron corridas electrofor谷-ticas en condiciones reductoras, los geles no te idos fueron transferidos a papel de nitrocelulosa (NC), los anticuerpos unidos a papel de NC se marcaron con Prote赤na A de Staphylococcus aureus (SPA) y las bandas inmunorreactivas se observaron por autorradiograf赤a. En WB se encontr車 que los animales de las tres especies, infectados con T. spiralis reconocen varios componentes antig谷nicos con rango de peso molecular de 29-97 kDa con predominio de un triplete de 50 kDa, 47 kDa y 45 kDa en el 100% de los sueros estudiados In this study, the detection of dominant antigens of Trichinella spiralis in three experimenta modeis (rat, mouse and rabbit), was carried out by Western Blot autoradiography (WB). Healthy animals were considered as controls. Experimenta animals were infected with infective larvae of T. spiralis: Animals were bleed before inoculation and at the fourth week post infection. The tri-chinella soluble antigens (AST) were obtained from muscles of experimentally infected rats. The AST were separated by reducing electrophoresis. Proteins in not stained gels were transferred to nitrocellulose paper sheets (NC), antibodies linked to NC were labeled with Staphylococcus aureus protein A SPA y (SPA) and the immune reactive bands were observed by autoradiography. By WB, T. spiralis infected animals recognized several antigenic componentes with a molecular weight ranging from 29 to 97 kDa, with dominant bands at 50, 47 and 45 kDa observed in 100% of studied sera. All controls were negative
%K Trichinella spiralis
%K Trichinellosis
%K Western Blot
%K Antigens
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-07202001000100002