%0 Journal Article
%T Activated mammalian target of rapamycin is a potential therapeutic target in gastric cancer
%A Da-zhi Xu
%A Qi-rong Geng
%A Ying Tian
%A Mu-yan Cai
%A Xin-juan Fang
%A You-qing Zhan
%A Zhi-wei Zhou
%A Wei Li
%A Ying-bo Chen
%A Xiao-wei Sun
%A Yuan-xiang Guan
%A Yuan-fang Li
%A Tong-yu Lin
%J BMC Cancer
%D 2010
%I BioMed Central
%R 10.1186/1471-2407-10-536
%X The expression of p-mTOR was detected in specimens of 181 gastric cancers who underwent radical resection (R0) by immunohistochemistry. The correlation of p-mTOR expression to clinicopathologic features and survival of gastric cancer was studied. We also determined the inhibition effect of RAD001 on tumor growth using BGC823 and AGS human gastric cancer cell lines.Immunostaining for p-mTOR was positive in 93 of 181 (51.4%) gastric cancers, closely correlated with lymph node status and pTNM stage. Patients with p-mTOR positive showed significantly shorter disease-free survival (DFS) and overall survival (OS) rates than those with p-mTOR-negative tumors in univariable analyses, and there was a trend toward a correlation between p-mTOR expression and survival in multivariable analyses. RAD001 markedly inhibited dose-dependently proliferation of human gastric carcinoma cells by down-regulating expression of p70s6k, p-p70s6k, C-myc, CyclinD1 and Bcl-2, up-regulating expression of P53.In gastric cancer, p-mTOR is a potential therapeutic target and RAD001 was a promising treatment agent with inducing cell cycle arrest and apoptosis by down-regulating expression of C-myc, CyclinD1 and Bcl-2, up-regulating expression of P53.Gastric carcinoma is the fourth most common cancer and the second leading cause of cancer-related death worldwide, including 1 million new cases per year throughout the world [1]. About 60% of new cases of gastric cancer occur in eastern Asia [2], especially in China. Surgical resection is the most effective treatment for gastric cancer and the efficacy of chemotherapy remains limited [3]. Many studies have indicated that the depth of invasion and the number of metastatic lymph nodes are the most important powerful predictors of survival for gastric cancer patients [4,5]. For example, in previous studies [6,7], we have found positive lymph node ratio is an independent prognostic indicator after D2 resection and intraperitoneal chemotherapy may be benefici
%U http://www.biomedcentral.com/1471-2407/10/536