%0 Journal Article
%T Genetic variation in the genome-wide predicted estrogen response element-related sequences is associated with breast cancer development
%A Jyh-Cherng Yu
%A Chia-Ni Hsiung
%A Huan-Ming Hsu
%A Bo-Ying Bao
%A Shou-Tung Chen
%A Giu-Cheng Hsu
%A Wen-Cheng Chou
%A Ling-Yueh Hu
%A Shian-Ling Ding
%A Chun-Wen Cheng
%A Pei-Ei Wu
%A Chen-Yang Shen
%J Breast Cancer Research
%D 2011
%I BioMed Central
%R 10.1186/bcr2821
%X This case-control study involved 815 patients of Asian descent with incident breast cancer and 821 healthy female controls. A total of 13,737 ERE sites in the whole genome predicted by a genome-wide computational algorithm were blasted with single-nucleotide polymorphism (SNP) sequences. Twenty-one SNPs located within 2,000 bp upstream or within introns 1 and 2 of putative genes and with a minor allele frequency greater than 5% were identified and genotyped. Frequencies of SNPs were compared between cases and controls to identify SNPs associated with cancer susceptibility.A significant combined effect of rs12539530, an ERE SNP in intron 2 of NRCAM which codes for a cell adhesion molecule, and SNPs of ESR1, the gene coding for ER, on breast cancer risk was found. Interestingly, this combined effect was more significant in women who had experienced a longer period of lifetime estrogen exposure, supporting a hormonal etiology of this SNP in breast tumorigenesis.Our findings provide support for a role of genetic variation in ERE-ESR1 in determining susceptibility of breast cancer development.The roles of estrogen receptor ¦Á (ER¦Á) in initiating tumor development in breast cancer, regulating progression and determining therapeutic protocols and efficacy are well documented [1-3]. Although ER¦Á can be activated in an estrogen-independent manner, the classical activation mechanism involves ER¦Á binding to estrogen and other coactivator proteins to form the estrogen-bound ER complex, which functions as a transcriptional regulator [4,5]. The DNA-binding domain of ER¦Á binds to estrogen response elements (EREs) in the promoter region of estrogen-responsive genes, activating or repressing their transcription and consequently mediating physiological or tumorigenic effects. Given that sequence variants, such as single-nucleotide polymorphisms (SNPs), located in the promoters of genes have the potential to affect the protein (transcription factor)-DNA (promoter) interaction, resultin
%U http://breast-cancer-research.com/content/13/1/R13