%0 Journal Article
%T Diagnosis of lung cancer in individuals with solitary pulmonary nodules by plasma microRNA biomarkers
%A Jun Shen
%A Ziling Liu
%A Nevins W Todd
%A Howard Zhang
%A Jipei Liao
%A Lei Yu
%A Maria A Guarnera
%A Ruiyun Li
%A Ling Cai
%A Min Zhan
%A Feng Jiang
%J BMC Cancer
%D 2011
%I BioMed Central
%R 10.1186/1471-2407-11-374
%X By using quantitative reverse transcriptase PCR analysis, we first determine plasma expressions of five miRNAs in a training set of 32 patients with malignant SPNs, 33 subjects with benign SPNs, and 29 healthy smokers to define a panel of miRNAs that has high diagnostic efficiency for lung cancer. We then validate the miRNA panel in a testing set of 76 patients with malignant SPNs and 80 patients with benign SPNs.In the training set, miR-21 and miR-210 display higher plasma expression levels, whereas miR-486-5p has lower expression level in patients with malignant SPNs, as compared to subjects with benign SPNs and healthy controls (all P ¡Ü 0.001). A logistic regression model with the best prediction was built on the basis of miR-21, miR-210, and miR-486-5p. The three miRNAs used in combination produced the area under receiver operating characteristic curve at 0.86 in distinguishing lung tumors from benign SPNs with 75.00% sensitivity and 84.95% specificity. Validation of the miRNA panel in the testing set confirms their diagnostic value that yields significant improvement over any single one.The plasma miRNAs provide potential circulating biomarkers for noninvasively diagnosing lung cancer among individuals with SPNs, and could be further evaluated in clinical trials.Lung cancer is the second most common cancer and the number one cancer killer in the USA and worldwide [1]. Lung cancer is often diagnosed at an advanced stage. The 5-year survival rate for stage IV lung cancer is only 10%, whereas approximately 80% for stage IA disease [2,3]. These statistics provide the primary rationale to improve lung cancer screening and early detection [4]. Chest X-ray and sputum cytology have been used for lung cancer screening. However, the sensitivity was low [2,3]. Several randomized trials in the USA and Europe have been carried out with the hope that high-resolution CT imaging can detect lung cancer earlier, much as screening has done for breast and colorectal cancer [5,6].
%U http://www.biomedcentral.com/1471-2407/11/374