%0 Journal Article
%T Caffeic acid phenethyl ester decreases acute pneumonitis after irradiation in vitro and in vivo
%A Miao-Fen Chen
%A Peter C Keng
%A Paul-Yang Lin
%A Cheng-Ta Yang
%A Shuen-Kuei Liao
%A Wen-Cheng Chen
%J BMC Cancer
%D 2005
%I BioMed Central
%R 10.1186/1471-2407-5-158
%X We compared the effects of CAPE on cytotoxicity and intracellular oxidative stress in normal lung fibroblast and a lung cancer cell line. For in vivo analysis, whole thorax radiation (single dose 10 Gy and 20 Gy) was delivered to BALB/c male mice with or without CAPE pretreatment. NF- kappaB activation and the expression levels of acute inflammatory cytokines were evaluated in mice after irradiation.The in vitro studies showed that CAPE cause no significant cytotoxicity in normal lung as compared to lung cancer cells. This is probably due to the differential effect on the expression of NF-kappa B between normal and malignant lung cells. The results from in vivo study showed that CAPE treatment decreased the expression of inflammatory cytokines including IL-1 alpha and beta, IL-6, TNF-alpha and TGF- beta, after irradiation. Moreover, histological and immunochemical data revealed that CAPE decreased radiation- induced interstitial pneumonitis and TGF-beta expression.This study suggests that CAPE decreases the cascade of inflammatory responses induced by thoracic irradiation without causing toxicity in normal lung tissue. This provides a rationale for combining CAPE and thoracic radiotherapy for lung cancer treatment in further clinical studies.Lung cancer is the leading cause of cancer death worldwide. Radiotherapy is an important modality of cancer treatment. Because radiation pneumonitis is a major obstacle to increasing the radiation dose, it is important to determine how the incidence of radiation- induced complication might be decreased and how the dose that normal lung can tolerate might be increased. Caffeic acid phenethyl ester (CAPE) is a phenolic antioxidant, an active anti-inflammatory component of propolis [1-3]. Several studies and our previous study have shown that the compound elicits several interesting biological functions including inducing apoptosis in various tumor cell types and anti-inflammatory properties [4-6]. Since reactive oxygen species (RO
%U http://www.biomedcentral.com/1471-2407/5/158