%0 Journal Article
%T Changes in gene expression induced by Micro-Immunotherapy
%A Capieaux Etienne
%A Donat de Groote
%A Pierre Dorfman
%A Maurice Jeaner
%J International Journal of High Dilution Research
%D 2012
%I Universidade Estadual Paulista
%X Background: Metabolic syndrome (MS) is a metabolic disorder associated with obesity, type-II diabetes, and  ¡éa   ¡°low grade inflammation ¡éa    , with the concomitant increased risk of cardiovascular events. As a chronic inflammatory process, MS results in a dysregulation of the cytokine profile. 2L    INFLAM, a Micro-immunotherapy (MI) medication formulated with highly diluted cytokines, is currently prescribed in Belgium for inflammatory diseases and potentially may be helpful for MS patients. Aims: To investigate the impact of 2L    INFLAM on selected gene expression markers (mRNA) in patients suffering from MS, in addition to biological and clinical parameters. Methodology: Four well characterized MS adult patients with stabilized body-weight were advised to take one capsule of 2L    INFLAM per day (by sublingual-oral route) for 6 months (composition in table 1). Concomitantly to biological and clinical examination, genes expression status was assessed by a DNA microarray technology (Oxygen ¡éa?  ¡é) comprising 200 genes involved mainly in oxidative stress and inflammation. Whole blood collection was performed before and after treatment (3-6 months) and mRNA levels measured. Gene expression was classified in 3 series (normally expressed, up or down-regulated) and genes related to diabetes predisposition were scored by using a proprietary Diascore (Probiox). Results: Before MI medication, a significant percentage of dysregulated genes (median: 16.3%) as well as a positive Diascore (median: 1.6) were noticed. Impressive correction of dysregulated genes (reaching 90% for one patient) was observed after 3 months of treatment (median: 2.3%) in addition to an improvement of Diascore in 3 MS patients out of 4 (median: 0.5). During the same period, both clinical and biological parameters remained unchanged. Conclusions: MS patients showing a high level of gene dysregulation efficiently normalized after 3 months of 2L    INFLAM (64%-90%), suggesting a biological regulatory effect of MI and a potential benefit of this medication for diabetic patients. Up and down-deregulated gene profiles were specific for each patient and not related to cytokine components of the formula. These preliminary data support the  ¡éa   ¡°domino effect ¡éa     of MI sequential formula to restore in depth the immune homeostasis. DNA microarray technology may represent a promising tool for new provings as well as for biochemical comprehension of the  ¡éa   ¡°in vivo ¡éa     effectiveness of highly diluted immune messengers. Table 1: 2L    INFLAM composition Compounds Dilutions Interleukin-1 (
%K Metabolic syndrome
%K Gene expression
%K Micro-arrays
%K Micro-immunotherapy
%K 2L    INFLAM
%U http://www.feg.unesp.br/~ojs/index.php/ijhdr/article/view/560