%0 Journal Article
%T miRNAs in lung cancer - Studying complex fingerprints in patient's blood cells by microarray experiments
%A Andreas Keller
%A Petra Leidinger
%A Anne Borries
%A Anke Wendschlag
%A Frank Wucherpfennig
%A Matthias Scheffler
%A Hanno Huwer
%A Hans-Peter Lenhof
%A Eckart Meese
%J BMC Cancer
%D 2009
%I BioMed Central
%R 10.1186/1471-2407-9-353
%X We synthesized 866 human miRNAs and miRNA star sequences as annotated in the Sanger miRBase onto a microarray designed by febit biomed gmbh. Using the fully automated Geniom Real Time Analyzer platform, we analyzed the miRNA expression in 17 blood cell samples of patients with non-small cell lung carcinomas (NSCLC) and in 19 blood samples of healthy controls.Using t-test, we detected 27 miRNAs significantly deregulated in blood cells of lung cancer patients as compared to the controls. Some of these miRNAs were validated using qRT-PCR. To estimate the value of each deregulated miRNA, we grouped all miRNAs according to their diagnostic information that was measured by Mutual Information. Using a subset of 24 miRNAs, a radial basis function Support Vector Machine allowed for discriminating between blood cellsamples of tumor patients and controls with an accuracy of 95.4% [94.9%-95.9%], a specificity of 98.1% [97.3%-98.8%], and a sensitivity of 92.5% [91.8%-92.5%].Our findings support the idea that neoplasia may lead to a deregulation of miRNA expression in blood cells of cancer patients compared to blood cells of healthy individuals. Furthermore, we provide evidence that miRNA patterns can be used to detect human cancers from blood cells.Lung cancer is the leading cause of cancer death worldwide [1]. Its five-year survival rate is among the lowest of all cancer types and is markedly correlated to the stage at the time of diagnosis [2]. Using currently existing techniques, more than two-thirds of lung cancers are diagnosed at late stages, when the relative survival rate is low [3]. This reality calls for the search of new biomarkers that are able to catch lung cancer while it is still small and locally defined.MicroRNAs (miRNA) are a recently discovered class of small non-coding RNAs (17-24 nucleotides) [4]. Due to their function as regulators of gene expression they play a critical role both in physiological and in pathological processes, such as cancer [5-8]. This fa
%U http://www.biomedcentral.com/1471-2407/9/353