%0 Journal Article
%T MicroRNA-143 down-regulates Hexokinase 2 in colon cancer cells
%A Lea H Gregersen
%A Anders Jacobsen
%A Lisa B Frankel
%A Jiayu Wen
%A Anders Krogh
%A Anders H Lund
%J BMC Cancer
%D 2012
%I BioMed Central
%R 10.1186/1471-2407-12-232
%X To gain insight into the role of miR-143 in colon cancer, we used a microarray-based approach in combination with seed site enrichment analysis to identify miR-143 targets.As expected, transcripts down-regulated upon miR-143 overexpression had a significant enrichment of miR-143 seed sites in their 3'UTRs. Here we report the identification of Hexokinase 2 (HK2) as a direct target of miR-143. We show that re-introduction of miR-143 in the colon cancer cell line DLD-1 results in a decreased lactate secretion.We have identified and validated HK2 as a miR-143 target. Furthermore, our results indicate that miR-143 mediated down-regulation of HK2 affects glucose metabolism in colon cancer cells. We hypothesize that loss of miR-143-mediated repression of HK2 can promote glucose metabolism in cancer cells, contributing to the shift towards aerobic glycolysis observed in many tumors.
%K miR-143
%K Colon cancer
%K Hexokinase 2
%K Glycolysis
%U http://www.biomedcentral.com/1471-2407/12/232/abstract