%0 Journal Article
%T Gossypol Analysis in Bt and Non-Bt Cotton Seed Extracts by High-Performance Liquid Chromatography (HPLC)
%A R.Chandrashekar
%A Karunakar Rao Kudle
%A P.Jyothi Chaitanya
%A N.Lakshmi Bhavani
%J International Journal of Herbal Medicine
%D 2013
%I AkiNik Publications
%X Present investigation on gossypol content of various seed extracts of Bt and non-Bt of cotton varieties was conducted through an optimized High-Performance Liquid Chromatography (HPLC) on a C18 column (4.6 mm ¡Á 250 mm, 5 mm particle) with methanol¨C0.5% acetic acid aqueous solution, 90: 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] < 10%, both intra-day and inter-day. The results revealed slightly differences among the Bt and non-Bt varieties of cotton in gossypol content including the Gossypium hirusutum (non-Bt) and Bt varieties of cotton.
%K Cotton seed
%K Gossypol
%K High-Performance Liquid Chromatography (HPLC).
%U http://florajournal.com/vol1issue2/june2013/15.1.pdf