%0 Journal Article
%T A 7 gene expression score predicts for radiation response in cancer cervix
%A Thangarajan Rajkumar
%A Neelakantan Vijayalakshmi
%A Kesavan Sabitha
%A Sundersingh Shirley
%A Ganesharaja Selvaluxmy
%A Mayil Bose
%A Lavanya Nambaru
%J BMC Cancer
%D 2009
%I BioMed Central
%R 10.1186/1471-2407-9-365
%X Archival RNA samples from 15 patients who had achieved complete remission and stayed disease free for more than 36 months (No Evidence of Disease or NED group) and 10 patients who had failed radical radiotherapy (Failed group) were included in the study. The RNA were amplified, labelled and hybridized to Stanford microarray chips and analyzed using BRB Array Tools software and Significance Analysis of Microarray (SAM) analysis. 20 genes were selected for further validation using Relative Quantitation (RQ) Taqman assay in a Taqman Low-Density Array (TLDA) format. The RQ value was calculated, using each of the NED sample once as a calibrator. A scoring system was developed based on the RQ value for the genes.Using a seven gene based scoring system, it was possible to distinguish between the tumours which were likely to respond to the radiotherapy and those likely to fail. The mean score ¡À 2 SE (standard error of mean) was used and at a cut-off score of greater than 5.60, the sensitivity, specificity, Positive predictive value (PPV) and Negative predictive value (NPV) were 0.64, 1.0, 1.0, 0.67, respectively, for the low risk group.We have identified a 7 gene signature which could help identify patients with cervical cancer who can be treated with radiotherapy alone. However, this needs to be validated in a larger patient population.Cervical cancer is a preventable but the most common cancer among Indian women and second most common cancer among women worldwide [1,2]. More than 500,000 women are expected to develop the disease every year worldwide. The tragedy is worsened by most of the women presenting in locally advanced disease as well. Human Papilloma virus has been identified as an important factor in the development of cervical cancer, but it alone is not sufficient and requires additional events [3]. Cervical cancer progresses through initial dysplastic or pre-malignant stages before becoming invasive. The evolution to an invasive cancer may take up to 15 years o
%U http://www.biomedcentral.com/1471-2407/9/365