%0 Journal Article
%T Possibility of multivariate function composed of plasma amino acid profiles as a novel screening index for non-small cell lung cancer: a case control study
%A Jun Maeda
%A Masahiko Higashiyama
%A Akira Imaizumi
%A Tomio Nakayama
%A Hiroshi Yamamoto
%A Takashi Daimon
%A Minoru Yamakado
%A Fumio Imamura
%A Ken Kodama
%J BMC Cancer
%D 2010
%I BioMed Central
%R 10.1186/1471-2407-10-690
%X The amino-acid concentrations in venous blood samples from pre-treatment NSCLC patients (n = 141), and age-matched, gender-matched, and smoking status-matched controls (n = 423), were measured using liquid chromatography and mass spectrometry. The resultant study data set was subjected to multiple logistic regression analysis to identify amino acids related with NSCLC and construct the criteria for discriminating NSCLC patients from controls. A test data set derived from 162 patients and 3,917 controls was used to validate the stability of the constructed criteria.The plasma amino-acid profiles significantly differed between the NSCLC patients and the controls. The obtained model (including alanine, valine, isoleucine, histidine, tryptophan and ornithine concentrations) performed well, with an area under the curve of the receiver-operator characteristic curve (ROC_AUC) of >0.8, and allowed NSCLC patients and controls to be discriminated regardless of disease stage or histological type.This study shows that plasma amino acid profiling will be a potential screening tool for NSCLC.Recently, computer-aided systems for data mining, for example by multivariate analysis, are now readily available and have shown promising results when applied to metabolic profiling for diagnostic purposes [1,2]. Currently, several applications of metabolome analysis based on machine learning for human cancer diagnosis using peripheral blood or urine were demonstrated [3-10].Among metabolites, the amino-acid balance in patients with various diseases often differs from that maintained in healthy individuals, as a result of metabolic changes. Amino acids are considered to be central compounds within metabolic networks. The blood serves as the medium linking the metabolic processes in the different organs of the human body. Human amino-acid metabolism in the blood has been monitored clinically for >30 years. Fischer's ratio, which is defined as the balance between branched-chain amino acids (BC
%U http://www.biomedcentral.com/1471-2407/10/690