%0 Journal Article
%T Identification of ¦Á(1,6)fucosylated proteins differentially expressed in human colorectal cancer
%A Laura Muinelo-Romay
%A Susana Villar-Portela
%A Elisa Cuevas
%A Emilio Gil-Mart¨ªn
%A Almudena Fern¨¢ndez-Briera
%J BMC Cancer
%D 2011
%I BioMed Central
%R 10.1186/1471-2407-11-508
%X In the current work we combined a LCA-affinity chromatography with SDS-PAGE and mass spectrometry in order to identify ¦Á(1,6)fucosylated proteins differentially expressed in colorectal cancer. This strategy allowed the identification of a group of ¦Á(1,6)fucosylated proteins candidates to be involved in CRC malignancy.The majority of the identified proteins take part in cell signaling and interaction processes as well as in modulation of the immunological response. Likewise, we confirmed the increased expression of GRP94 in colorectal cancer tissue and the significant down-regulation of the IgGFcBP expression in tumour cells.All these results validate the importance of core-fucosylated proteins profile analysis to understand the mechanisms which promote cancer onset and progression and to discover new tumour markers or therapeutic targets.Colorectal carcinoma (CRC) is one of the most frequent tumours in the Western world. Although early stages are successfully treatable, many cases are undiagnosed until late stages when the prognosis is poor [1]. Therefore, the identification and verification of proteins that have a functional role in the patho-physiology of CRC remains an important goal to discover new biomarkers for diagnosis, prognosis and follow-up, as well as to find therapeutic targets.Glycosylation plays fundamental roles in controlling various biological processes such as embryonic development, immune response and cell-cell interactions involving sugar-sugar or sugar-protein specific recognition [2]. An universal hallmark of cancer cells is the change in their glycosylation phenotype, with several effects on this tumour cells behavior [3]. In this sense, glycosylation analysis has become an important target for proteomic research and has reached great interest to understand the molecular events associated with tumour development and progression.One of the most frequent alterations in the normal glycosylation pattern observed during carcinogenesis is the enhan
%K Colorectal cancer
%K Glycoproteins
%K ¦Á(1
%K 6)fucosyltransferase
%K GRP94
%K IgGFcBP
%U http://www.biomedcentral.com/1471-2407/11/508