%0 Journal Article
%T Detection of Porcine Circovirus Type 2 (PCV2) in Mosquitoes from Pig Farms by PCR
%A Xiaohong Yang
%A Lidan Hou
%A Jing Ye
%A Qigai He and Shengbo Cao*
%J Pakistan Veterinary Journal
%D 2012
%I University of Agriculture, Faisalabad
%X To investigate, whether mosquitoes could be potential vectors that maintain or transmit PCV2, 59 mosquito samples were collected from suspicious PCV2-infected pig farms in Hubei and Anhui province, China. Total DNA from these mosquitoes was extracted and then tested for presence of PCV2 nucleic acid by PCR. Four (6.78%) samples showed the positive result. Subsequently, the positive PCR product was cloned into pEASY-T1 vector and sequenced. Sequence analysis displayed that homology between the PCR products and PCV2 strains were more than 94%. These results demonstrate that PCV2 nucleic acid exists in these mosquitoes, which suggests that mosquitoes could serve as mechanical transmission vectors of PCV2.
%K Mosquitoes
%K PCV2
%K Polymerase chain reaction
%K (PCR)
%K Vector
%U http://pvj.com.pk/pdf-files/32_1/134-135.pdf