%0 Journal Article
%T Clinical protection against caprine herpesvirus 1 genital infection by intranasal administration of a live attenuated glycoprotein E negative bovine herpesvirus 1 vaccine
%A Julien Thiry
%A Maria Tempesta
%A Michele Camero
%A Elvira Tarsitano
%A Beno£¿t Muylkens
%A Fran£¿ois Meurens
%A Etienne Thiry
%A Canio Buonavoglia
%J BMC Veterinary Research
%D 2007
%I BioMed Central
%R 10.1186/1746-6148-3-33
%X The vaccine was inoculated intranasally twice three weeks apart followed by a subsequent CpHV-1 intravaginal challenge which is the natural route of infection in three goats. To analyse the safety and the efficacy of this marker vaccine, two groups of three goats served as controls: one immunised with a virulent CpHV-1 and one uninoculated until the challenge. Goats were clinically monitored and all sampling procedures were carried out in a blind manner. The vaccine did not induce any undesirable local or systemic reaction and goats did not excrete gE-negative BoHV-1. After challenge, a significant reduction in disease severity was observed in immunised goats. Moreover, goats immunised with either gE-negative BoHV-1 or CpHV-1 exhibited a significant reduction in the length and the peak of viral excretion. Antibodies neutralising both BoHV-1 and CpHV-1 were raised in immunised goats.Intranasal application of a live attenuated gE-negative BoHV-1 vaccine is able to afford a clinical protection and a reduction of virus excretion in goats challenged by a CpHV-1 genital infection.The subfamily Alphaherpesvirinae includes a cluster of closely related ruminant viruses with bovine herpesvirus 1 (BoHV-1) as prototype [1]. BoHV-1, a major cattle pathogen, is typically responsible of infectious bovine rhinotracheitis (IBR) causing severe economic losses in livestock [2]. Since its isolation, several conventional vaccines have been developed. These vaccines usually prevented clinical signs and reduced the amount of excreted viruses. However, there was still a need for improvements in order to use them in control and/or eradication programmes [3]. Therefore, BoHV-1 marker vaccines comprising attenuated or killed mutants with a deletion in one of the non-essential genes (gE) were developed and eradication campaigns were initiated in many European countries. They have proven their safety and efficacy in the target bovine species since they are efficacious at reducing disease severi
%U http://www.biomedcentral.com/1746-6148/3/33