%0 Journal Article
%T First detection, isolation and molecular characterization of infectious salmon anaemia virus associated with clinical disease in farmed Atlantic salmon (Salmo salar) in Chile
%A Marcos G Godoy
%A Alejandra Aedo
%A Molly JT Kibenge
%A David B Groman
%A Carmencita V Yason
%A Horts Grothusen
%A Angelica Lisperguer
%A Marlene Calbucura
%A Fernando Avenda£¿o
%A Marcelo Imil¨¢n
%A Miguel Jarpa
%A Frederick SB Kibenge
%J BMC Veterinary Research
%D 2008
%I BioMed Central
%R 10.1186/1746-6148-4-28
%X In mid-June 2007, an Atlantic salmon marine farm site located in central Chilo¨¦ Island in Region X of Chile registered a sudden increase in mortality following recovery from an outbreak of Pisciricketsiosis, which rose to a cumulative mortality of 13.6% by harvest time. Based on the clinical signs and lesions in the affected fish, and laboratory tests performed on the fish tissues, a confirmatory diagnosis of ISA was made; the first time ISA in its classical presentation and for the first time affecting farmed Atlantic salmon in Chile. Rapid sequencing of the virus-specific RT-PCR products amplified from the fish tissues identified the virus to belong to the European genotype (Genotype I) of the highly polymorphic region (HPR) group HPR 7b, but with an 11-amino acid insert in the fusion glycoprotein, and ability to cause cytopathic effects (CPE) in CHSE-214 cell line, characteristics which make it distinct from common European Genotype ISAV isolates from Europe and North America.In conclusion, the present work constitutes the first report of a case of ISA in farmed Atlantic salmon in Chile. The clinical signs and lesions are consistent with the classical descriptions of the disease in marine-farmed Atlantic salmon in the Northern hemisphere. The outbreak was caused by ISAV of European genotype (or Genotype I) of HPR 7b but distinct from common European Genotype ISAV isolates.Infectious salmon anaemia (ISA) is a viral disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), which belongs to the genus Isavirus, family Orthomyxoviridae [1]. In the Northern hemisphere, the first registered outbreak of ISA was in 1984 in Atlantic salmon "parr", on the southwestern coast of Norway [2]. Subsequently the disease was reported in Canada in 1996 [3], in Scotland in 1998 [4], in Faeroe Islands in 1999 [5], and in Maine, USA, in 2000 [6]. The clinical disease in farmed Atlantic salmon is characterized by variable mortality ranging from 0 to 50% with asc
%U http://www.biomedcentral.com/1746-6148/4/28