%0 Journal Article
%T Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina
%A Silvina S Maidana
%A Mar¨ªa F Ladelfa
%A Sandra E P¨¦rez
%A Patricia M Lom¨®naco
%A Mar¨ªa P Del M¨¦dico Zajac
%A Anselmo Ode¨®n
%A Javier Viera
%A Gustavo Combessies
%A Norberto Fondevila
%A Mar¨ªa Palacios
%A Julien Thiry
%A Beno£¿t Muylkens
%A Etienne Thiry
%A Sonia A Romera
%J BMC Veterinary Research
%D 2011
%I BioMed Central
%R 10.1186/1746-6148-7-8
%X Seventeen field strains of BoHV-5 isolated from 1984 to now were confirmed by differential PCR and subjected to restriction endonuclease analysis (REA). Viral DNA was cleaved with BstEII which allows the differentiation among subtypes a, b and non a, non b. According to the REA with BstEII, only one field strain showed a pattern similar to the Argentinean A663 strain (prototype of BoHV-5b). All other isolates showed a clear pattern similar to the Australian N569 strain (prototype of BoHV-5a) consistent with the subtypes observed in Brazil, the other South-American country where BoHV-5 is known to be prevalent. The genomic region of subtype b responsible for the distinct pattern was determined and amplified by PCR; specifically a point mutation was identified in glycoprotein B gene, on the BstEII restriction site, which generates the profile specific of BoHV-5b.This is the first report of circulation of BoHV-5a in Argentina as the prevailing subtype. Therefore the circulation of BoHV-5b was restricted to a few years in Argentina, speculating that this subtype was not able to be maintained in the bovine population. The mutation in the gB gene is associated with the difference in the restriction patterns between subtypes "a" and "b".Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are closely related alphaherpesviruses infecting cattle [1,2]. The major difference between BoHV-5 and BoHV-1 is the distinct ability of the former to cause neurological disease in cattle [3,4]. In a natural infection via the respiratory tract, BoHV-1 and BoHV-5 replicate similarly in the nasal mucosa but they differ in their neuroinvasiveness [5,6]. BoHV-1 neuroinvasion usually does not go further than the first order neuron located in the trigeminal ganglia, where the latent infection is established, whereas BoHV-5 is able to infect different regions of the brain [7-9].The BoHV-5 genome is 137,821 base pairs (bp) long and is approximately 2,000 bp longer than the BoHV-1 genome, with a G + C b
%U http://www.biomedcentral.com/1746-6148/7/8