%0 Journal Article
%T MicroRNA Target Identification¡ªExperimental Approaches
%A Aida Martinez-Sanchez
%A Chris L. Murphy
%J Biology
%D 2013
%I MDPI AG
%R 10.3390/biology2010189
%X MicroRNAs (miRNAs) are small non-coding RNA molecules of 21¨C23 nucleotides that control gene expression at the post-transcriptional level. They have been shown to play a vital role in a wide variety of biological processes and dysregulated expression of miRNAs is observed in many pathologies. Understanding the mechanism of action and identifying functionally important mRNA targets of a specific miRNA are essential to unravelling its biological function and to assist miRNA-based drug development. This review summarizes the current understanding of the mechanistic aspects of miRNA-mediated gene repression and focuses on the different approaches for miRNA target identification that have been proposed in recent years.
%K miRNA
%K gene expression
%K miRISC
%K miRNA target identification
%K RIP-Seq
%K HITS-CLIP
%K HT-Seq
%U http://www.mdpi.com/2079-7737/2/1/189