%0 Journal Article
%T Molecular characterisation of the caprine (Capra hircus) lymphocyte function-associated antigen-1 alpha subunit-encoding cDNA
%A Thomas Fett
%A Laurent LM Zecchinon
%A Etienne A Baise
%A Daniel JM Desmecht
%J BMC Veterinary Research
%D 2005
%I BioMed Central
%R 10.1186/1746-6148-1-4
%X The Capra hircus CD11a-encoding cDNA was sequenced and compared with its human, murine, rat, bovine and ovine counterparts. Despite some focal differences, it shares all the main characteristics of its known mammalian homologues.Therefore, along with the caprine CD18-encoding cDNA, which has been available for a few months, the sequence data revealed here will allow the Capra hircus LFA-1 expression in vitro as a tool to explore the specificities of inflammation in the caprine species.Lymphocyte function-associated antigen-1 (LFA-1, ¦ÁL¦Â2, CD11a/CD18) is a member of the ¦Â2-integrin subfamily of cell surface receptors. Integrins consist of a 120 to 180 kDa ¦Á subunit (CD11a in this case) and a 90 to 110 kDa ¦Â subunit that are noncovalently associated single-pass transmembrane proteins [1]. The bulk of each integrin subunit is extracellular, where it typically functions as a receptor for extracellular matrix molecules or as a counterreceptor for surface proteins of apposed cells [2]. The heterodimer CD11a/CD18 is expressed on all leukocytes and mediates high affinity adhesion to a variety of cell types that express one or more of the ¦Â2-integrins ligands, intercellular adhesion molecules (ICAM-1 to -5) [3-5]. The adhesion process mediated is a critical step of a wide range of immunological activities, including cytolysis of target cells, cross-interaction and cross-stimulation between lymphocytes, phagocytosis of complement-coated targets, neutrophils clearance from inflamed tissue, and the regulation of leukocyte traffic between the bloodstream and tissues [6-9]. As the relevance of the goat model for studying leukocyte traffic, diapedesis and pathologic tissue infiltration is well established in such important areas as mastitis [10-13] or lentivirus-associated diseases [14-16], increasing our knowledge about caprine ¦Â2 integrins is of great importance to offer new possibilities for research and to provide additional insights into those fields. Along with the caprine C
%U http://www.biomedcentral.com/1746-6148/1/4