%0 Journal Article
%T Effect of Cations and Chemicals on the Activity of Partially Purified Cellulase from Tomato (Lycopersicon esculentum Mill) Fruits Deteriorated by Aspergillus flavus Linn
%A A.A. Ajayi
%A A.O. Adejuwon
%A O.K. Awojobi
%A P.O. Olutiola
%J Pakistan Journal of Nutrition
%D 2007
%I 
%X Within ten days of incubation, freshly ripe tomato fruits (Ibadan local variety) obtained from a local market in Ile-Ife, Osun State, Nigeria had completely deteriorated and proteins which possessed cellulolytic activity were released. The enzyme was partially purified by a combination of ammonium sulphate precipitation, molecular exclusion chromatography and ion-exchange chromatography. The enzyme was stimulated to varying degrees by Ca++, Mg++, Na+ and K+ but was inhibited by ethylene diamine tetraacetic acid, 2, 4-dinitrophenol and mercuric chloride.
%K Tomato fruits
%K cellulolytic activity
%K ion-exchange
%K chromatography
%K Aspergillus flavus
%U http://docsdrive.com/pdfs/ansinet/pjn/2007/198-200.pdf