%0 Journal Article
%T DETECTION OF DNA DAMAGE IN OBESE CARDIOVASCULAR DISEASE PATIENTS AND COMPARISON BETWEEN GENDERS: A CASE-CONTROL STUDY
%A GANDHI G.
%A KAUR J. and MAHAJAN N.
%J International Journal of Genetics
%D 2011
%I Bioinfo Publications
%X Background- Obesity and cardiovascular disease (CVD) with the increased stress/ inflammation may cause DNA damage posing risk for malignancy hence assessment of DNA damage gains importance as a pre-cancerous biomarker.Methods-The alkaline Single Cell Gel Electrophoresis assay was carried out in peripheral blood leukocytes of obese-CVD patients (n=28; 14 males and 14 females) and matched controls (n=13; 7 males and 6 females). Multiple regression and analysis of variance were performed for effect of confounding/specific factors on DNA damage and the Student¡¯s t-lest to compare differential values for factors associated with obesity-CVD and DNA damage.Results-In patients, genomic damage was significantly higher (p<0.05): damage frequency (59.21% in males, 60.14% in females), mean DNA migration length (37.53¡À4.74 ¦Ìm, 19.16¡À2.60¦Ìm, respectively) in comparison to control data with significantly (p<0.01) increased damage in male patients. The attributes of obese-CVD patients and controls matched except for Body Mass Index (BMI), weight, waist circumference (WC) and waist hip ratio (WHR). Multivariate analysis of variance and multiple regression analysis revealed association of genetic damage with waist and hip circumferences in male patients. The t-test revealed that age, BMI, WHR, WC, cardiovascular disease sub-sets and drug usage had differential significance for genetic damage in female patients. Male patients with arrhythmia had significantly higher DNA damage as those on atenolol-rampril-furosemide therapy.Conclusion- The significantly increased DNA damage in obese-CVD patients may be from the nature of CVD, treatment duration, drug combinations and adiposity and gains importance as it imples a higher risk for age -related diseases and cancer.
%K SCGE/comet assay
%K Damage Frequency
%K Damage Index
%U SCGE/comet assay, Damage Frequency, Damage Index