%0 Journal Article
%T Mesenchymal stem cells with rhBMP-2 inhibits the growth of canine osteosarcoma cells
%A Rose Grassi Rici
%A Dayane Alcantara
%A Paula Fratini
%A Cristiane Wenceslau
%A Carlos Ambr¨Žsio
%A Maria Miglino
%A Durvanei Maria
%J BMC Veterinary Research
%D 2012
%I BioMed Central
%R 10.1186/1746-6148-8-17
%X We evaluated the regenerative potential of in vitro treatment with rhBMP-2 and found that both osteogenic induction and tumor regression occur in stem cells from canine bone marrow. rhBMP-2 inhibits the proliferation capacity of OST cells by mechanisms of apoptosis and tumor suppression mediated by p53.We propose that rhBMP-2 has great therapeutic potential in bone marrow cells by serving as a tumor suppressor to increase p53 and the pro-apoptotic proteins Bad and Bax, as well as by increasing the activity of phosphorylated caspase 3.Canine bone marrow mesenchymal stem cells associated with rhBMP2 in canine osteosarcoma treatment: "in vitro" studyOsteosarcoma is as a primary bone cancer common in dogs. Frequently, osteosarcoma affects the limb bones of large-sized dogs over 15 kg at an average age of 7 years [1]. In 75% of cases, osteosarcoma affects either the appendicular skeleton [2] or the pelvic and thoracic limbs, and in the remaining 25%, it affects the axial skeleton or the flat bones [3,4]. Generally, males have a higher incidence of osteocarcoma than females [2], with the exception of the St. Bernard, Rottweiler, and Danish breeds, in which females are most affected [5,6]. Osteosarcoma cells induce platelet aggregation, which facilitates metastasis formation. Platelet aggregation and metastasis most commonly occur in the lung [7]. Platelet aggregation promotes the establishment of tumor cell aggregates, which could serve as a bridge between the tumor cells and the vascular surfaces [6].A primary extraskeletal osteosarcoma has a metastatic rate that ranges from 60 to 85% in dogs and an average life expectancy after surgery of 26-90 days, which varies according to the location where the metastasis occurs [4].Metastasis is the most common cause of death in dogs with osteosarcoma, and 90% of dogs either die or are euthanized due to complications associated with lung metastases. Therefore, chemotherapy is used to increase the long-term survival of dogs with ost
%K Osteosarcoma
%K rhBMP-2
%K Mesenchymal stem cell
%K Canine
%U http://www.biomedcentral.com/1746-6148/8/17