%0 Journal Article
%T Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
%A SARA ROJAS-DOTOR
%A JULIA P¨¦REZ-RAMOS
%A JUAN ANTONIO GIM¨¦NEZ-SCHERER
%A FRANCISCO BLANCO-FAVELA
%J Biological Research
%D 2009
%I Sociedad de Biolog¨ªa de Chile
%X Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-l , IFN-¦Ã, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1 cytokine (IFN-¦Ã) and increased Th2 cytokines (IL-4 and IL-10). The co-expression of the cytokine and chemokine receptors IFN-¦Ã/CCR5 and IL-1 /CCR5 was inhibited by PMA+MLIF and Th2 co-expression was increased. MLIF effects varied depending on the conditions. MLIF alone activated the Th1 and Th2 cytokines and cytokine/receptor expression; however, PMA+MLIF increased the expression of Th2 but inhibited it in Th1.
%K Amebic hepatic abscess (AHA)
%K CC chemokine receptors (CCR4
%K CCR5)
%K monocyte locomotion inhibitory factor (MLIF)
%K Entamoeba histolytica (E. histolytica)
%K 1-phorbol-12 myristate-13 acetate (PMA)
%K interleukin (IL)
%K T helper 1 lymphocyte (Th1)
%K T helper 2 lymphocyte Th2)
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003