%0 Journal Article
%T Modified protocol for in vivo imaging of wild-type mouse retina with customized miniature spectral domain optical coherence tomography (SD-OCT) device
%A Ferguson Lee R
%A Balaiya Sankarathi
%A Grover Sandeep
%A Chalam Kakarla V
%J Biological Procedures Online
%D 2012
%I BioMed Central
%R 10.1186/1480-9222-14-9
%X This protocol outlines and evaluates a modified scanning procedure for a customized spectral domain optical coherence tomography (SD-OCT) imaging apparatus within the wild-type C57Bl/6 mouse posterior segment. This modified protocol allows for the capture of a 50 degree field of view spanning 3 mm by 3 mm perimeter with the optic disc as the central point. By utilizing this scanning protocol a more reliable measurement of retinal thickness can be achieved outside the fluctuating region of the optic disc. This protocol, when applied to this high resolution device, enables non-invasive in vivo histological imaging and biometric assessment of the various layers of the rodent posterior segment within a 20 ¨C 30 min procedural time-frame. This protocol could establish a standardized method for evaluating morphological changes, with this commercial SDOCT device, when assessing longitudinal disease pathophysiology and treatment response in mouse models for future vision science research.
%K Spectral domain optical coherence tomography
%K Customized
%K Retinal imaging
%K Mouse
%U http://www.biologicalproceduresonline.com/content/14/1/9