%0 Journal Article
%T Solution-based targeted genomic enrichment for precious DNA samples
%A Aiden E Shearer
%A Michael S Hildebrand
%A Richard JH Smith
%J BMC Biotechnology
%D 2012
%I BioMed Central
%R 10.1186/1472-6750-12-20
%X We developed a solution-based TGE method for downstream Illumina sequencing in a non-automated workflow, adding standard Illumina barcode indexes during the post-hybridization amplification to allow for sample pooling prior to sequencing. The method utilizes Agilent SureSelect baits, primers and hybridization reagents for the capture, off-the-shelf reagents for the library preparation steps, and adaptor oligonucleotides for Illumina paired-end sequencing purchased directly from an oligonucleotide manufacturing company.This solution-based TGE method for Illumina sequencing is optimized for small- or medium-sized laboratories and addresses the weaknesses of standard protocols by reducing the amount of input DNA required, increasing capture yield, optimizing efficiency, and improving reproducibility.
%K Targeted genomic enrichment
%K Sequence capture
%K Massively parallel sequencing
%K Illumina
%U http://www.biomedcentral.com/1472-6750/12/20/abstract