%0 Journal Article
%T The Effects of Culture Solution pH on Root Formation, Root Growth and Root Morphology in Trifoliate Orange Budded with Satsuma Mandarin
%A Pear Mohammad
%A Masaya Shiraishi
%J Pakistan Journal of Biological Sciences
%D 1999
%I Asian Network for Scientific Information
%X The formation rate, elongation and periclinal growths, and the morphology of roots in trifoliate orange budded with satsuma mandarin under culture solution pH 4.5-5.6, 6.0-6.3 and 7.5-7.6 were studied to know the favorable range of culture media acidity for this plant. Root formation was earlier and maximum and the elongation growth was highest at pH 4.5-5.6 followed by pH 6.0-6.3 and 7.5-7.6. The periclinal growth also increased in terms of stelar and overall diameters along with elongation under pH 4.5-5.6 but periclinal development became slower or remained constant at pH 6.0-6.3 and 7.5-7.6. Normal sloughing of older rootcap cells leaving sharply pointed root tips and the presence of distinctly visible intact epidermal cells were observed under pH 4.5-5.6 at all exposures. While at pH 6.0-6.3, moderately severe removal of rootcap cells was evident at short exposure followed by root tip injuries and root surface ridging on prolonged induction. The severe removal of rootcap cells under pH 7.5-7.6 became apparent in a short period and root tip injuries occurred subsequently on prolonged exposure. Epidermal peel off and root surface ridging were prominent due to the destruction of epidermal cells and gradual root death with the increase of exposure time. This study suggests that the favorable range of growing media pH for trifoliate orange budded with satsuma mandarin lies between 4.5-5.6.
%U http://docsdrive.com/pdfs/ansinet/pjbs/1999/14-20.pdf