%0 Journal Article
%T MORPHOLOGICAL OBSERVATIONS ON THE TESTES OF Physalaemus cuvieri (AMPHIBIA, ANURA) ESTUDIO MORFOL車GICO DE LOS TEST赤CULOS DE Physalaemus cuvieri (AMPHIBIA, ANURA)
%A Classius de Oliveira
%A Cristiani Zanetoni
%A Rodrigo Zieri
%J Revista chilena de anatom赤a
%D 2002
%I Scientific Electronic Library Online
%X In general, the testes in the anurans are paired ovoid organs, constituted by a mass of seminiferous structures surrounded by a layer of fibrous connective tissue, which holds a germ epithelium with characteristic cellular types. This study describes the testicular histological architecture and anatomy, as well as the organization and morphology of germ cells. Five male samples, from the Botucatu area (S o Paulo State, Brazil), of Physalaemus cuvieri (Leptodactylidae) were used. After macroscopic analyses and obtainment of the testicular fragments, the material was submitted to the histological routine to inclusion in paraffin and coloration with haematoxylin/eosin. A rare peculiarity is the presence of numerous pigment-containing cells randomly distributed in the albuginea tunic and testicular interstitium, giving the testis a dark brown coloration. In the germ tissue the spermatogonia I are biggest spermatogenetic cells. With the cellular differentiation and proliferation, succeded the other cellular types (spermatogonia II, spermatocytes I and II, spermatids I and II, and spermatooza) with a cystic organization, that is, groups of cells associated with Sertoli cells, forming the spermatogenetic cysts or spermatocysts. The spermatogenetic lineage cells were differentiated and identified according to the cellular and cystic morphology. Generalmente, los test赤culos de anuros constituyen 車rganos ovoides, pares, formados por masa de estructuras semin赤feras, envueltas en una capa de tejido conjuntivo fibroso, que contiene un epitelio germinativo provisto de tipos celulares caracter赤sticos. En este trabajo se describe la anatom赤a y la arquitectura histol車gica de los test赤culos, as赤 como tambi谷n la organizaci車n y morfolog赤a de las c谷lulas germinativas. Se utilizaron cinco ejemplares machos de la especie Physalaemus cuvieri (Leptodactylidae), provenientes de la zona de Botucatu (S o Paulo, Brasil). Tras el an芍lisis macrosc車pico y la obtenci車n de los fragmentos testiculares, el material se someti車 a las t谷cnicas histol車gicas rutinarias para su inclusi車n en parafina y coloraci車n con hematoxilina/eosina. Se constat車 como rara peculiaridad la presencia de numerosas c谷lulas con pigmento distribuidas de forma aleatoria en la t迆nica albug赤nea e intersticio testicular, las cuales otorgan al test赤culo una coloraci車n marr車n oscura. En el tejido germinativo las espermatogonias I son las mayores c谷lulas espermatogen谷ticas. En la secuencia de la diferenciaci車n y proliferaci車n celulares, se siguen los dem芍s tipos de c谷lulas (espermatogonias II, espermatocitos I y II, esper
%K Anura
%K Leptodactylidae
%K Physalaemus cuvieri
%K Anura
%K Leptodactylidae
%K Physalaemus cuvieri
%K Test赤culo
%K Espermatog谷nesis
%K Testis
%K Spermatogenesis
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-98682002000300005