%0 Journal Article
%T ULTRASTRUCTURAL ALTERATIONS IN SUBMANDIBULAR GLANDS OF RATS (Rattus norvegicus) SUBMITTED TO EXPERIMENTAL CHRONIC ALCOHOLISM ALTERACIONES ULTRAESTRUCTURALES DE LAS GL芍NDULAS SUBMANDIBULARES DE RATONES (Rattus norvegicus) SOMETIDOS A ALCOHOLISMO CR車NICO EXPERIMENTAL
%A L. F Tirapelli
%A D. P. C Tirapelli
%A O. J Tamega
%J Revista chilena de anatom赤a
%D 2002
%I Scientific Electronic Library Online
%X The objective of this study was to analyze the submandibular glands of rats (Rattus norvegicus) submitted to experimental chronic alcoholism at electronic transmission microscopy. The experiment was programmed for a 15-month period, and in every five months, three animals from each group were sacrificed. Twenty-seven male rats, with average weight of 300g, were used and divided into three experimental groups with the following characteristics: 1) alcoholic: received ethanol, daily, at 6% for 15 months; 2) isocaloric: received diluted sucrose at a concentration of 87.14 g/L, corresponding energetically to the caloric content of the alcoholic group; and 3) control: received tap water "ad libitum" as liquid diet. The animals were fed with the same amount of Purina ration as a conventional weekly solid diet. It was observed several ultrastructural alterations in the alcoholic animals mainly in seromucous cells: dilation and enlargement of Golgi complex cisterns; the presence of digestive vacuoles, a large number of electronlucent vesicles and an increase in intercellular space, an increase between basolateral interdigitation cell and an enlargement of connective tissue. It was observed at some cells of the intercalary ducts at a lower part of lateral membranes, only after 15-month treatment, the presence of wide and clear vesicles with electronlucent material located among cell secretion granules of granular ducts. No alterations were observed in the animals of the control and isocaloric groups as well as the other parts of glandular parenchyma of animals of the alcoholic group El objetivo de este estudio fue analizar las gl芍ndulas submandibulares de ratones (Rattus norvegicus) sometidos al alcoholismo cr車nico experimental, utilizando el microscopio electr車nico de transmisi車n. El experimento fue programado para un per赤odo de 15 meses. Cada 5 meses, 3 animales de cada grupo fueron sacrificados. Utilizamos 27 ratones (machos) adultos, con peso medio de 400g los cuales fueron divididos en tres grupos experimentales: 1) Alcoh車lico, el cual recibi車 etanol diluido al 6% por 15 meses; 2) Isocal車rico que recibi車 sacarosa diluida en concentraci車n de 87,14 g/L, correspondiendo energ谷ticamente al contenido cal車rico del grupo alcoh車lico, y 3) Control, el cual recibi車 agua de grifo "ad libitum"como dieta l赤quida. Los animales de todos los grupos fueron alimentados con una dieta s車lida establecida semanalmente, que consisti車 en id谷ntica raci車n de Purina. Observamos varias alteraciones ultraestructurales, principalmente, en las c谷lulas seromucosas: dilataci車n y ensanchami
%K Gl芍ndulas submandibulares
%K Alteraci車n ultraestructural
%K Etanol
%K Rat車n
%K Ultrastructural alteration
%K Ethanol
%K Submandibular gland
%K Rats
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