%0 Journal Article
%T Genotype networks, innovation, and robustness in sulfur metabolism
%A Jo£żo F Matias Rodrigues
%A Andreas Wagner
%J BMC Systems Biology
%D 2011
%I BioMed Central
%R 10.1186/1752-0509-5-39
%X We show that metabolic genotypes with the same phenotype form large connected genotype networks - networks of metabolic networks - that extend far through metabolic genotype space. How far they reach through this space depends linearly on the number of super-essential reactions. A super-essential reaction is an essential reaction that occurs in all networks viable in a given environment. Metabolic networks can differ in how robust their phenotype is to the removal of individual reactions. We find that this robustness depends on metabolic network size, and on other variables, such as the size of minimal metabolic networks whose reactions are all essential in a specific environment. We show that different neighborhoods of any genotype network harbor very different novel phenotypes, metabolic innovations that can sustain life on novel sulfur sources. We also analyze the ability of evolving populations of metabolic networks to explore novel metabolic phenotypes. This ability is facilitated by the existence of genotype networks, because different neighborhoods of these networks contain very different novel phenotypes.We show that the space of metabolic genotypes involved in sulfur metabolism is organized similarly to that of carbon metabolism. We demonstrate that the maximum genotype distance and robustness of metabolic networks can be explained by the number of superessential reactions and by the sizes of minimal metabolic networks viable in an environment. In contrast to the genotype space of macromolecules, where phenotypic robustness may facilitate phenotypic innovation, we show that here the ability to access novel phenotypes does not monotonically increase with robustness.In any biological system, genotypes contain the information needed to make phenotypes. The relationship between genotype and phenotype is also known as a genotype-phenotype map [1]. The ability to analyze different kinds of biological systems computationally has allowed a detailed characterization
%U http://www.biomedcentral.com/1752-0509/5/39