%0 Journal Article
%T Similar temperature dependencies of glycolytic enzymes: an evolutionary adaptation to temperature dynamics?
%A Luisa Ana B Cruz
%A Marit Hebly
%A Giang-Huong Duong
%A Sebastian A Wahl
%A Jack T Pronk
%A Joseph J Heijnen
%A Pascale Daran-Lapujade
%A Walter M van Gulik
%J BMC Systems Biology
%D 2012
%I BioMed Central
%R 10.1186/1752-0509-6-151
%X Saccharomyces cerevisiae was grown under different temperature regimes and glucose availability conditions. These included glucose-excess batch cultures at different temperatures and glucose-limited chemostat cultures, subjected to fast linear temperature shifts and circadian sinoidal temperature cycles. An observed temperature-independent relation between intracellular levels of glycolytic metabolites and residual glucose concentration for all experimental conditions revealed that it is the substrate availability rather than temperature that determines intracellular metabolite profiles. This observation corresponded with predictions generated in silico with a kinetic model of yeast glycolysis, when the catalytic capacities of all glycolytic enzymes were set to share the same normalized temperature dependency.From an evolutionary perspective, such similar temperature dependencies allow cells to adapt more rapidly to temperature changes, because they result in minimal perturbations of intracellular metabolite levels, thus circumventing the need for extensive modification of enzyme levels.Growth and survival of microorganisms is strongly affected by environmental variables such as temperature, nutrient and oxygen availability, pH and osmolarity. Since, in natural environments, these parameters are highly dynamic, microorganisms have to cope with fluctuating, often non-optimal growth conditions. Suboptimal growth temperatures have major impacts on cell physiology including decreasing membrane fluidity and a reduced efficiency of protein synthesis and folding [1-3]. In addition, the catalytic capacity of each enzyme in the cell decreases when the temperature is lowered. This temperature impact can, in many cases, be described by an Arrhenius equation [4].In the past decade, the response of the mesophilic yeast Saccharomyces cerevisiae to suboptimal temperatures has been the focus of several studies [5-7]. Interest in this subject is motivated by the biotechnological app
%K Glycolysis
%K Kinetic modelling
%K Metabolomics
%K Saccharomyces cerevisiae
%K Temperature dynamics
%U http://www.biomedcentral.com/1752-0509/6/151