%0 Journal Article
%T Application of disease-associated differentially expressed genes ¨C Mining for functional candidate genes for mastitis resistance in cattle
%A Schwerin Manfred
%A Czernek-Sch£¿fer Diana
%A Goldammer Tom
%A Kata Srinivas R
%J Genetics Selection Evolution
%D 2003
%I BioMed Central
%R 10.1186/1297-9686-35-s1-s19
%X In this study the mRNA differential display method was applied to identify mastitis-associated expressed DNA sequences based on different expression patterns in mammary gland samples of non-infected and infected udder quarters of a cow. In total, 704 different cDNA bands were displayed in both udder samples. Five hundred-and-thirty two bands, (75.6%) were differentially displayed. Ninety prominent cDNA bands were isolated, re-amplified, cloned and sequenced resulting in 87 different sequences. Amongst the 19 expressed sequence tags showing a similarity with previously described genes, the majority of these sequences exhibited homology to protein kinase encoding genes (26.3%), to genes involved in the regulation of gene expression (26.3%), to growth and differentiation factor encoding genes (21.0%) and to immune response or inflammation marker encoding genes (21.0%). These sequences were shown to have mastitis-associated expression in the udder samples of animals with and without clinical mastitis by quantitative RT-PCR. They were mapped physically using a bovine-hamster somatic cell hybrid panel and a 5000 rad bovine whole genome radiation hybrid panel. According to their localization in QTL regions based on an established integrated marker/gene-map and their disease-associated expression, four genes (AHCY, PRKDC, HNRPU, OSTF1) were suggested as potentially involved in mastitis defense.
%K mastitis
%K expressed sequence tag
%K gene expression
%K cattle
%K RH mapping
%U http://www.gsejournal.org/content/35/S1/S19