%0 Journal Article
%T Enhancement in VitaminB12 Production By Mutant Strains of Propionibacterium freudenreichii
%A Mir Naiman Ali
%A Mazharuddin Khan Mohd.
%J International Journal of Engineering Science and Technology
%D 2011
%I Engg Journals Publication
%X In the present study vitaminB12 production was carried out with parental as well as mutant strains of Propionibcterium freudenreichii. Physical mutagenesis was performed by three different doses of U.V. irradiation- 200, 300 and 400erg/mm2and five different irradiation time periods were selected- 30sec, 60sec, 90sec, 120 sec and 150sec. Obtained 15 mutant strains were used to produce vitaminB12 by anaerobic and aerobic fermentation techniques at 300C, 320C and 340C. 200 erg/mm2 U.V. dose and short duration exposurehaven¡¯t developed any desirable character in organism but mutants which were exposed to 400 erg/mm2 for 60 seconds developed desirable (resistant to propionic acid) character. High yield of vitmainB12 was produced at 300C by mutants of P. freudenreichii.
%K VitaminB12
%K physical mutagenesis
%K U.V. radiation
%K propionic acid
%K bioassay.
%U http://www.ijest.info/docs/IJEST11-03-06-051.pdf